| 吴巧芬，夏 科，赵志国，马晓雅，仇 硕.华重楼组培快繁技术研究[J].广东农业科学,2022,49(5):10-18 |
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华重楼组培快繁技术研究 |
| Establishment of Tissue Culture and Rapid PropagationSystem for Paris polyphylla var. chinensis |
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| DOI：10.16768/j.issn.1004-874X.2022.05.002 |
| 中文关键词: 华重楼 消毒 不定芽 诱导 增殖 生根和结鳞茎 |
| 英文关键词: Paris polyphylla var. chinensis sterilization adventitious bud induction propagation rooting and bulb setting |
| 基金项目:广西重点研发计划项目（桂科 AB18294026）；广西科学院基本业务费项目（2018YJJ909）；广西科学院园艺植物种质资源创新及利用创新团队启动经费项目（CQZ-E-1919）；广西植物研究所基本业务费项目（桂植业 22006） |
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| 中文摘要: |
| 【目的】建立华重楼组培快繁技术体系。【方法】以华重楼幼苗为试材，研究外植体预处理方式
和消毒方法，筛选适宜诱导的外植体，筛选不定芽诱导、增殖以及生根和结鳞茎最佳培养基。【结果】华重楼
外植体先经过 0.5% 多菌灵浸泡 2 h，再利用 75% 乙醇浸泡 45 s 和 0.1% HgCl2 消毒 10 min，污染率最低（1.67%），
诱导的不定芽长势最好。高 5~10 cm、带根系的华重楼幼苗或去掉茎叶的幼苗是最适外植体，不定芽诱导最佳
培养基为 WPM+2.0 mg/L ZT+0.1 mg/L NAA+30 g/L 蔗糖，诱导率达 98.33%，萌发的新芽生长更快；不定芽增殖
最适培养基为 MS+2.0 mg/L 6-BA+1.0 mg/L IAA+30 g/L 蔗糖，培养 90 d 后增殖系数为 3.75；不定芽生根和结鳞茎
最适培养基为 WPM+2.0 mg/L ZT+0.1 mg/L NAA+30 g/L 蔗糖，生根和结鳞茎率达 96.67%，根系数 3.84 条，根长
4.15 cm，鳞茎直径 0.86 cm。生根苗 3—5 月份移栽到基质为泥炭土︰珍珠岩︰河沙 =2 ∶ 1 ∶ 1 的组合物中成活
率最高，为 30%。【结论】建立了适合华重楼幼苗组培快繁的技术体系，为华重楼的资源保护及规模化生产优
质种苗奠定了基础。 |
| 英文摘要: |
| 【Objective】The study was conducted to establish a tissue culture and rapid propagation system for
Paris polyphylla var. chinensis.【Method】The seedlings of P. polyphylla var. chinensis were selected as materials
in this experiment. Different pretreatment and sterilization method for explants were studied. And the optimum
explants, the suitable medium for adventitious buds induction, propagation, rooting and bulb setting were also screened.
【Result】The contamination rate reached the lowest (1.67%) and the new buds grew best after the explants was dipped
into 0.5% carbendazim for 2 h and then dipped into 75% ethanol for 45 s, and 0.1% HgCl2
for 10 min. The best medium
for adventitious buds induction was WPM+2.0 mg/L ZT+0.1 mg/L NAA+30 g/L sucrose, the induction rate reached up to 98.33% and new buds grew fast and well when the seedlings or the seedlings without leaves and stems were selected as explants,
which were 5-10 cm high with roots. The optimal medium for adventitious buds propagation was MS + 2.0 mg/L 6-BA+1.0
mg/L IAA+30 g/L sucrose and the propagation coefficient reached 3.75 times after being cultured for 90 d. While the optimal
medium for adventitious buds rooting and bulb culture was WPM+2.0 mg/L ZT+0.1 mg/L NAA+30 g/L sucrose, the rooting and
bulb setting rate reached 96.67%, the root number was 3.84, the root length was 4.15 cm and the bulb diameter was 0.86 cm.
The best medium for transplanting and domestication of seedlings was peat soil ∶ perlite ∶ river sand = 2 ∶ 1 ∶ 1 on March
to April and the survival rate reached 30%.【Conclusion】A tissue culture and rapid propagation system for P. polyphylla
var. chinensis seedlings was established, which laid a foundation for resource conservation and mass production of seedlings of
P. polyphylla var. chinensis. |
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