| 李 洁，王 飞，徐凌川.基于高通量分析泰山白首乌根际土壤细菌群落结构[J].广东农业科学,2022,49(5):75-84 |
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基于高通量分析泰山白首乌根际土壤细菌群落结构 |
| Bacterial Community Structure of Rhizosphere Soil ofCynanchum bungei Decne Based on High-throughput Analy |
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| DOI：10.16768/j.issn.1004-874X.2022.05.009 |
| 中文关键词: 泰山白首乌 根际土壤 细菌 微生物群落 高通量测序技术 |
| 英文关键词: Cynanchum bungei Decne rhizosphere soil bacteria microbial community high-throughput sequencing technology |
| 基金项目:山东省科技攻关项目（2010GSF275）；山东省教育厅科研项目（J08LH10） |
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| 中文摘要: |
| 【目的】了解泰山白首乌根际土壤微生物群落结构和组成，为充分利用根际微生物资源，优选根
际促生菌、提高仿野生种植技术提供理论依据及科学支撑。【方法】采用 Illumina MiSeq 高通量测序技术，分析
测定 3 个产地 2 个根茎深度的泰山白首乌根际土壤细菌群落的丰富度和多样性。【结果】测序结果显示，6 个泰
山白首乌根际土壤样品共得到细菌 5 404 个 OTUs，分属于 22 门 63 纲 98 目 170 科 274 属，其中样品 LW20 的优
势菌门为酸杆菌门 Acidobacteria，相对丰度为 24.76%；样品 LW10、LX10、LX20、CQ10、CQ20 的优势菌门均
为变形菌门 Proteobacteria，相对丰度分别为 57.89%、46.00%、38.22%、27.29%、31.05%；样品 LW10 的优势属
为假单胞菌属 Pseudomonas，相对丰度为 22.35%；样品 LW20 的优势属为 Spartobacteria-genera-incertae-sedis，
相对丰度为 21.37%；样品 LX10 的优势属为土壤红杆菌属 Solirubrobacter，相对丰度为 5.57%；样品 LX20 的优
势属为鞘氨醇单胞菌属 Sphingomonas，相对丰度为 4.31%；样品 CQ10 的优势属为 norank-Acidobacteria-Gp4，
相对丰度为 9.58%；样品 CQ20 的优势属为 Streptophyta，相对丰度为 9.02%。【结论】综合比较，样品 CQ10 的
细菌群落丰度和多样性最高，Chao 和 Ace 指数分别为 1 150.41 和 1 102.51；样品 LX10 的细菌群落分布最均匀，
Shannoneven 指数为 0.8315；随着地下根茎深度加深，细菌微生物群落的丰度和多样性降低，不同产地和根茎深
度样品间土壤细菌群落存在显著差异性。 |
| 英文摘要: |
| 【Objective】The study was conducted to understand the structure and composition of rhizosphere soil
microbial community of Cynanchum bungei Decne, which provided theoretical basis and scientific support for making full
use of rhizosphere microbial resources, optimizing rhizosphere growth-promoting bacteria and improving imitation wild
planting technology.【Method】With Illumina MiSeq high-throughput sequencing technology, the richness and diversity of
rhizosphere soil bacterial communities of Cynanchum bungei Decne were analyzed and determined.【Result】According
to the sequencing results, a total of 5 404 OTUs were obtained from 6 rhizosphere soil samples, belonging to 22 phyla, 63 classes, 98 orders, 170 families and 274 genera, among which the dominant phyla of LW20 was Acidobacteria, with a
relative abundance of 24.76%; the dominant phyla of LW10, LX10, LX20, CQ10 and CQ20 were Proteobacteria, with a relative
abundance of 57.89%, 46.00%, 38.22%, 27.29% and 31.05%, respectively; the dominant genus of LW10 was Pseudomonas,
with a relative abundance of 22.35%; the dominant genus of sample LW20 was Spartobacteria general incertae sedis, and
the relative abundance was 21.37%; the dominant genus of sample LX10 was Solirubrobacter, and the relative abundance was
5.57%; the dominant genus of LX20 was Sphingomonas, and the relative abundance was 4.31%; the dominant genus of CQ10
was Norank acidobacteria GP4, and the relative abundance was 9.58%; the dominant genus of sample CQ20 was Streptophyta,
and the relative abundance was 9.02%【Conclusion】Comprehensive comparison showed that the bacterial community
abundance and diversity of sample CQ10 were the highest, and Chao and Ace indexes were 1 150.41 and 1 102.51, respectively.
The bacterial community distribution of sample LX10 was the most uniform, and Shannoneven index was 0.8315. With the
deepening of underground rhizome depth, the abundance and diversity of bacterial microbial community decreased. There were
significant differences in soil bacterial communities among samples from different producing areas and rhizome depths. |
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