| 白农恩，刘李蕾，邓 巍，姜 林，杨晓燕.小生境范围内 3 种园林植物叶际细菌群落结构及多样性分析[J].广东农业科学,2022,49(6):43-50 |
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小生境范围内 3 种园林植物叶际细菌群落结构及多样性分析 |
| Analysis on Phyllosphere Bacterial Community Structureand Diversity of Three Garden Plants in Niche Area |
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| DOI：10.16768/j.issn.1004-874X.2022.06.006 |
| 中文关键词: 叶际微生物 细菌多样性 互作机制 高通量测序 植物健康管理 |
| 英文关键词: phyllosphere microorganism bacterial diversity interaction mechanism high-throughput sequencing plant
health management |
| 基金项目:国家自然科学基金（32160268）；云南省教育厅科学研究基金（2022Y887） |
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| 中文摘要: |
| 【目的】研究小生境下园林绿化植物叶际细菌群落结构及其多样性，了解小生境下相似环境条件
与植物自身特性对微生物群落的影响，为园林植物的健康管理及叶际细菌资源的挖掘提供参考。【方法】应用
高通量测序技术，探究大理大学校园 50 m 半径圆内朝向东边的 3 种主要园林植物 （枇杷树 Eriobotyra japonica、
广玉兰 Magnolia grandifl ora 和大青树 Ficus altissima）的叶际细菌群落结构及其多样性。【结果】 3 种园林植
物的叶际细菌多样性从高到低依次为枇杷树、广玉兰和大青树；不同植物的叶际细菌优势门类均为变形菌门
（Proteobacteria），第一优势细菌属不同，但排名前五的细菌属均为鞘氨醇单胞菌属（Sphingomonas）、甲基杆
菌属（Methylobacterium）、螺状菌属（Spirosoma）、薄层菌属（Hymenobacter）和无色菌属（Achromobacter）。
3 种植物的叶际细菌群落结构存在较大差异，但同一植物不同大小叶片间的细菌群落相似，其中，仅在枇杷树和
广玉兰叶片上分布的细菌群落门和属最多，表明常绿阔叶植物的叶际细菌群落相较于落叶阔叶植物更多样、复杂。
【结论】不同园林植物的叶际细菌群落组成和多样性存在较大差异， 植物自身特性是造成这种差异的首要原因，
植物叶际细菌 资源的挖掘有待加强。 |
| 英文摘要: |
| 【Objective】The study was conducted to explore the phyllosphere bacterial community structure and
diversity of landscaping plants in the niche, and to understand the effects of similar environmental conditions and plant
characteristics on microbial community in the niche, with a view to provide references for the health management of garden plants and the exploitation of phyllosphere bacterial resources.【Method】High-throughput sequencing technology was applied
to explore the bacterial community structure and diversity of Eriobotyra japonica, Magnolia grandifl ora and Ficus altissima
leaves facing east within 50 m radius circle of Dali University campus.【Result】Among the three garden plants, E. japonica
had the highest phyllosphere bacterial diversity, followed by M. grandifl ora and F. altissima. The dominant bacterial phylum of
phyllosphere bacteria in different plants was Proteobacteria, and the first dominant bacterial genus was different. However, the
top five dominant genera were Sphingopyxis, Methylobacterium, Spirosoma, Hymenobacter and Achromobacter. There were
significant differences in phyllosphere bacterial community structure among the three plants, but the bacterial communities
were similar among the leaves with different sizes of the same plant. Only E. japonica and M. grandiflora leaves had the
largest phylum and genus distribution, indicating that the phyllosphere bacterial communities of evergreen broad-leaved
plants was more diverse and complex than those of deciduous broad-leaved plants.【Conclusion】There were differences in
the composition and diversity of phyllosphere bacterial communities among different plants, and the primary reasons for these
differences were the characteristics of the plants themselves. Therefore, it is urgent to strengthen the researches on the utilization
of phyllosphere bacterial resources of plants. |
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