| 罗. 艺，杨淦麟，谭嘉娜，陈月桂，罗剑飘，杨俊贤.千里香离体再生体系的建立[J].广东农业科学,2022,49(7):26-32 |
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千里香离体再生体系的建立 |
| Establishment of In Vitro RegenerationSystem for Murraya paniculata |
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| DOI：10.16768/j.issn.1004-874X.2022.07.004 |
| 中文关键词: 千里香 种子 增殖 壮苗 生根 离体再生体系 |
| 英文关键词: Murraya paniculata seed proliferation strong seedling rooting in vitro regeneration system |
| 基金项目:建设广东省科学院分支机构项目（2017-2019） |
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| 中文摘要: |
| 【目的】千里香自然播种的繁殖方式无法满足大面积生产种植需求，通过建立高效的离体再生
体系为千里香组培种苗工厂化繁育奠定基础。【方法】以千里香成熟种子为外植体材料，研究不同激素浓度与
配比和不同有机物对千里香种子萌发、增殖培养、壮苗培养和生根培养阶段的影响，获得无菌组培苗，进行炼
苗移栽。【结果】千里香种子经 75% 酒精与 0.1% 升汞预处理后，最适萌发培养基为 MS+6-BA 0.5 mg/L，萌发
率为 62.5%；丛生芽继代增殖最适基本培养基为 MS 培养基；增殖培养最适培养基配方为 MS+6-BA 0.50 mg/L+
NAA 0.15 mg/L，增殖系数达 2.37；壮苗培养适宜培养基配方为 MS+ 马铃薯汁 30 g/L，40 d 株高增长 2.0 cm；
生根培养最适培养基配方为 1/2MS+IBA 0.6 mg/L+ 马铃薯汁 30 g/L+ 香蕉汁 30 g/L+ 活性炭 0.1 mg/L，生根率为
55.6%。驯化后将生根苗移栽于泥炭土∶黄心土∶珍珠岩∶钙镁磷肥（3 ∶ 2 ∶ 1 ∶ 0.2）的混合基质中，成活
率 85%。【结论】不同激素及浓度对千里香种子萌发和丛芽增殖过程影响显著，添加马铃薯汁和香蕉汁利于壮
苗和生根培养。 |
| 英文摘要: |
| 【Objective】The natural propagation mode for Murraya paniculata is sowing, which can not meet the
needs of extensive production of plantlets. The establishment of efficient in vitro regeneration system lays a foundation
for industrialized breeding of tissue culture seedlings for M. paniculata.【Method】Mature seeds of M. paniculata were
used as explants to study the effects of different concentrations and ratios of hormone and different organic matters on seed
germination, proliferation, strong seedling culture and rooting culture of M. paniculata. Then, sterile tissue culture seedlings
were obtained for transplanting.【Result】After the pretreatment with 75% alcohol and 0.1% HgCl2
, the most suitable
medium for seeds germination is MS+6-BA 0.5 mg/L, and the germination rate is 62.50%; the most appropriate basic
medium for subculture proliferation of cluster buds is the MS medium; the most suitable medium for multiplication culture is MS + 6-BA 0.50 mg/L + NAA 0.15 mg/L, and the multiplication coefficient is up to 2.37; the suitable medium for strong
seedling culture is MS + potato juice 30 g/L, and the plant height increase to 2.0 cm in 40 days; the optimal medium for rooting
culture is 1/2MS + IBA 0.6 mg/L + potato juice 30 g/L+ banana juice 30 g/L + AC 0.1 mg/L, and the rooting rate is 55.6%.
The survival rate of the rooting seedlings is 85% after domesticated and transplanted into the mixed matrix (peat soil: loess:
perlite: fertilizer of calcium, magnesium and phosphate, 3∶2∶1∶0.2).【Conclusion】Different hormones and concentrations have
significant effects on seed germination and cluster bud proliferation. Adding potato juice and banana juice are beneficial to the
growth of plantlets and rooting. |
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