| 钱玉磊，闫晋强，杨松光，刘文睿，谢大森，吴智明，江 彪.冬瓜抗氧化基因的克隆及其在非生物胁迫下的表达分析[J].广东农业科学,2022,49(7):33-41 |
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冬瓜抗氧化基因的克隆及其在非生物胁迫下的表达分析 |
| Analysis on Cloning and Expression of Oxidation ResistanceGenes in Wax Gourd Under Abiotic Stress |
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| DOI：10.16768/j.issn.1004-874X.2022.07.005 |
| 中文关键词: 冬瓜 抗氧化基因（OXR） 非生物胁迫 表达特征 |
| 英文关键词: wax gourd oxidation resistance genes (OXR) abiotic stress expression characteristic |
| 基金项目:广东省基础与应用基础研究基金 - 粤穗联合基金（2020A1515111138，2021A1515111214）；广东省农业科学院中青年学科带头人项目（R2020PY-JG003）；广东省农业科学院优秀博士项目（R2021YJ-YB2004） |
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| 中文摘要: |
| 【目的】鉴定、克隆冬瓜 BhiOXR 基因，明确其在非生物胁迫下的表达特征，为深入研究冬瓜
BhiOXR 基因的功能奠定基础。【方法】从冬瓜基因组鉴定 BhiOXR 基因家族成员，设计扩增 BhiOXR 基因 CDS
的全长引物，通过 RT-PCR 克隆 BhiOXR 基因。以低温（4℃）、高温（40℃）、盐胁迫（150 mmol/L NaCl 溶液）
和干旱胁迫（10%PEG6000 溶液）处理冬瓜高代自交系 B227 幼苗，利用 qRT-PCR 技术分析冬瓜 BhiOXR 基因
分别在上述 4 种非生物胁迫处理下（处理 0、4、8、12、24 h）的表达特征。【结果】鉴定到 6 个冬瓜 BhiOXR
成员，并进行全长基因克隆和生物信息学分析。qRT-PCR 分析结果表明，BhiOXR1 响应低温、高温和干旱胁
迫，不响应盐胁迫；BhiOXR2a、BhiOXR2b 和 BhiOXR4 响应低温、盐和干旱胁迫，不响应高温胁迫；BhiOXR3
和 BhiOXR5 响应低温、高温和盐胁迫，不响应干旱胁迫。【结论】明确了冬瓜 BhiOXR 基因受低温、高温、盐
和干旱等非生物胁迫诱导表达，并且不同 BhiOXR 基因响应的非生物胁迫有差异，由此推测 6 个 BhiOXR 成员可
能在抵御不同非生物胁迫过程中所表现的抗氧化作用不尽相同，为进一步探究冬瓜 BhiOXR 基因的生物学功能
奠定基础。 |
| 英文摘要: |
| 【Objective】Identification and cloning of BhiOXR genes from wax gourd and clarification of its expression
characteristics under abiotic stresses would lay a foundation for further studying the function of BhiOXR genes in wax gourd.
【Method】BhiOXR gene family members from wax gourd reference genome were identified, full-length CDS primers were
designed to clone the BhiOXR genes of the wax gourd by RT-PCR. Seedlings of wax gourd inbred line B227 were treated with low temperature (4℃ ), high temperature (40℃ ), salt stress (150 mmol/L NaCl solution) and drought (10% PEG6000 solution).
The expression characteristics of BhiOXR genes under the above mentioned abiotic stresses treatment time: 0, 4, 8, 12, 24 h were
analyzed by using qRT-PCR.【Result】Six BhiOXR members were identified from wax gourd reference genome, and the fulllength genes were further cloned and bioinformatically analyzed. qRT-PCR analysis results showed that BhiOXR1 responded
to low temperature, high temperature and drought stress, but did not respond to salt stress. BhiOXR2a, BhiOXR2b and
BhiOXR4 responded to low temperature, salt and drought stresses but not to high temperature stress; BhiOXR3 and BhiOXR5
responded to low temperature, high temperature and salt stresses but not to drought stress.【Conclusion】It is clarified that
BhiOXR genes are induced by abiotic stresses as low temperature, high temperature, salt and drought, and different BhiOXR
genes have differences in response to abiotic stresses. Therefore, it is speculated that the six BhiOXR members may have
different antioxidant effects in the process of resisting different abiotic stresses, which lays a foundation for further exploring the
biological functions of BhiOXR genes in wax gourd. |
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