| 黄 元 1
，陈锦良 1
，黄育浩 2
，张端秀 2
，王晓虎 1
，杨德胜 2
，王 刚 1
，向 华 1.东莞某屠宰场猪链球菌检测及分离鉴定[J].广东农业科学,2022,49(10):118-126 |
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东莞某屠宰场猪链球菌检测及分离鉴定 |
| Detection, Isolation and Identification of Streptococcus suis in A Slaughterhouse in Dongguan |
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| DOI：10.16768/j.issn.1004-874X.2022.10.014 |
| 中文关键词: 猪链球菌 流行病学 抗生素敏感性试验 屠宰场 |
| 英文关键词: HUANG Yuan, CHEN Jinliang, HUANG Yuhao, ZHANG Duanxiu, WANG Xiaohu,YANG Desheng, WANG Gang,XIANG Hua |
| 基金项目:广东省重点领域研发计划项目（2019B020217002，2020B0202080004）；广东省现代农业产业技术体系生猪产业创新团队项目（2020KJ126）；广东省科技基础条件建设领域项目（2021B1212050021） |
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| 中文摘要: |
| 【目的】猪链球菌（Streptococcus suis，SS）是一种重要的猪病原菌，也是一种人兽共患病病
原体。其中猪链球菌 2 型（SS2）致病力最强，不但引起猪的急性败血症、脑膜炎、关节炎甚至死亡，也可
导致人的感染和死亡。为评估屠宰场猪链球菌的传播风险，对东莞某屠宰场进行猪链球菌的采样检测和分离
鉴定。【方法】采集 40 头猪的关节液和扁桃体进行 PCR 检测和猪链球菌分离，分离菌分别进行形态学和生
理生化鉴定、16S rDNA PCR 扩增、分型 PCR 检测和抗生素敏感性试验。【结果】 对 40 头猪的关节液和扁桃
体提取 DNA 进行 PCR 检测，在 40 份关节液中，猪链球菌阳性率为 22.5%，但未检出猪链球菌 2 型阳性；在
40 份扁桃体样品中，猪链球菌阳性率为 32.5%，其中猪链球菌 2 型阳性率为 7.5%。从 40 头猪的扁桃体和关
节液共 80 份样品中分离出 3 株猪链球菌， PCR 结果表明，3 株分离菌中 1 株为猪链球菌 4 型，基因型为 epfmrp+gdh+gapdh+fbps-orf2+sly-；其他 2 株为猪链球菌 9 型，基因型分别为 epf-mrp+gdh+gapdh+fbps-orf2+sly-
和 epf-mrp-gdh+gapdh+fbps-orf2+sly+。药敏试验表明，3 株猪链球菌菌株对青霉素 G、林可霉素、多粘菌素 B
和磺胺异恶唑耐药，对恩诺沙星、环丙沙星、大观霉素和阿莫西林敏感。【结论】该屠宰场猪群中存在猪链球
菌的隐性感染，提示存在猪链球菌 2 型感染人的风险。 |
| 英文摘要: |
| 【Objective】Streptococcus suis (SS) is an important porcine pathogen and a zoonotic pathogen. Among
various types, Streptococcus suis type 2 (SS2) has the strongest pathogenicity. It can not only cause acute sepsis, meningitis,
arthritis and even death in pigs, but also lead to human infection and death. In order to evaluate the transmission risks of SS in slaughterhouses, the sampling, detection, isolation and identification of SS were carried out in a slaughterhouse
in Dongguan.【Method】The synovial fluid and tonsils of 40 pigs were collected for PCR detection and SS isolation. The
isolated strains were used for morphology, physiology and biochemistry identification, 16S rDNA PCR amplification, typing
PCR detection and antibiotic sensitivity test.【Result】The nucleic acids extracted from the synovial fluid and tonsils of 40
pigs were detected by PCR. In 40 synovial fluids, the positive rate of SS was 22.5%, but no SS2 was detected. In 40 tonsil
samples, the positive rate of SS was 32.5%, in which the positive rate of SS2 was 7.5%. Three strains of SS were isolated from
80 tonsil and synovial fluid samples of 40 pigs. The PCR results showed that one of the three strains was SS type 4, and the
genotype was epf-mrp+gdh+gapdh+fbps-orf2+sly-; while the other two strains were SS type 9, and the genotypes were epfmrp+gdh+gapdh+fbps-orf2+sly-and epf-mrp-gdh+gapdh+fbps-orf2+sly+, respectively. The drug sensitivity test showed that
the three SS strains were resistant to penicillin G, lincomycin, polymyxin B and sulfaisoxazole, and sensitive to enrofloxacin,
ciprofloxacin, spectinomycin and amoxicillin.【Conclusion】The results suggest that there are a large number of recessive
infection of SS in the pigs of the slaughterhouse, and there is a risk of human infection by SS2. |
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