| 张艳彬 1,2，王素峰 2
，张健玲 3
，刘书欣 2
，邓俊劲 2
，王志林 2
，陈 庄 2.猪源发酵乳杆菌的筛选及其潜在益生性能[J].广东农业科学,2022,49(11):50-56 |
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猪源发酵乳杆菌的筛选及其潜在益生性能 |
| Screening of Lactobacillus Fermentum from Pigletsand Its Potential Probiotic Properties |
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| DOI：10.16768/j.issn.1004-874X.2022.11.006 |
| 中文关键词: 发酵乳杆菌 益生性能 耐酸 耐胆盐 病原菌拮抗 |
| 英文关键词: Lactobacillus fermentum probiotic property acid tolerance bile salt tolerance pathogen antagonism |
| 基金项目:广东省科技专项资金（“大专项 + 任务清单”）项目（江科〔2020〕182 号，江科〔2021〕183 号）；
肇庆市科技计划项目（2021N003） |
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| 中文摘要: |
| 【目的】对从健康仔猪粪便中筛选分离出一株发酵乳杆菌 的益生性能进行测试，为其在畜禽动物
养殖中的应用提供参考。【方法】采用 Rogosa SL 完全选择性培养基分离出 106 株乳酸菌，通过革兰氏染色、酸
和胆盐耐受初步筛选出 1 株潜在的饲用乳酸菌，经 16S rDNA 序列鉴定为发酵乳杆菌 LF1，并对其益生性能潜力
进行评估，包括抑菌活性 ( 针对大肠杆菌 K88、金黄色葡萄球菌、猪链球菌、沙门氏菌以及弧菌等 12 种病原菌 )、
对模拟胃液中酸性环境的耐受性、对胆盐的耐受性以及对小肠上皮细胞的粘附性。【结果】发酵乳杆菌 LF1 耐
酸和耐胆盐的能力较强，显示出较强的益生菌潜力，但 LF1 菌株对猪肠道上皮细胞的粘附能力不强。在生物拮
抗作用方面，发酵乳杆菌 LF1 的培养上清可以显著抑制多种常见动物病原菌生长。【结论】发酵乳杆菌 LF1 具
有较好的益生性能，通过进一步的动物实验评估后，可以作为应用于畜禽养殖业的益生菌候选菌株。 |
| 英文摘要: |
| 【Objective 】A strain of Lactobacillus fermentum was isolated from the feces of healthy piglets, and its
probiotic properties were tested in order to provide references for its applications in livestock and poultry breeding.【Method】
106 strains of lactic acid bacteria were isolated by using Rogosa SL medium, and one strain which was potential for feeding
was preliminarily screened by gram staining, acid and bile salt tolerance tests. The probiotic ability of strain LF1, which was
identified as L. fermentum LF1 by 16S rDNA sequencing, was evaluated, including its antagonistic activity (Escherichia
coli K88, Staphylococcus aureus, Streptococcus suis, Salmonella, and Vibrio, etc.), tolerance to the stimulated acidic
environment in stomach and bile acid, and adhesion to small intestinal epithelial cells.【Result】L. fermentum LF1 had strong tolerance to acid and bile salt, showing its strong probiotic potential. However, the LF1 strain showed low adhesion to pig
intestinal epithelial cells. In terms of antagonism, the culture supernatant of L. fermentum LF1 inhibited the growth of many
animal pathogens significantly.【Conclusion】L. fermentum LF1 had a good probiotics ability. After further experimental
evaluation on animals, it can be used as a probiotic candidate strain for livestock and poultry breeding industry. |
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