| 潘 勇1，张贝贝 2，周亚彬 2，胡 捷 1，景玉辉 1，李林林 1，黄友谊 3，王 剑 1.培养条件对雪茄烟优势菌株 ZLX10 生长的影响[J].广东农业科学,2023,50(2):11-19 |
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培养条件对雪茄烟优势菌株 ZLX10 生长的影响 |
| Effects of Culture Conditions on the Growth of Dominant Strain ZLX10 from Cigar |
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| DOI：10.16768/j.issn.1004-874X.2023.02.002 |
| 中文关键词: 雪茄优势菌株 菌株鉴定 莫海威芽孢杆菌 培养条件优化 生物量 |
| 英文关键词: cigar dominant strain strain identification Bacillus mojavensis optimization of culture conditions biomass |
| 基金项目:湖北中烟工业有限责任公司科技项目（2021JCYL3SX2B011） |
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| 中文摘要: |
| 【目的】微生物在雪茄烟叶发酵过程中起关键作用，为更好地提高雪茄烟品质，需要筛选雪茄优势菌并应用于雪茄烟叶发酵，为此需优化扩大优势菌株的生物量。【方法】对从雪茄烟中筛选出的一株优势菌株 ZLX10 进行 16S 测序、生理生化分析以及菌株鉴定，同时通过单因素和正交试验优化培养基成分（碳源、氮源和无机盐）及培养条件（接种量、装液量和种龄），以提高其生物量。【结果】经 16S rDNA 序列比对，菌株ZLX10 与莫海威芽孢杆菌（Bacillus mojavensis，MT043920.1）的序列同源性达到 99.86%，该菌株具有很强的多糖和蛋白大分子降解能力，可以鉴定为莫海威芽孢杆菌。经单因素和正交试验得到菌株 ZLX10 的最优培养基配方为碳源（蔗糖）50 g/L、氮源（酵母提取物）20 g/L、无机盐（MgSO4） 0.25 g/L，最佳培养条件为接种量 1%（V/V）、装液量为 250 mL 中装 30 mL、种龄 24 h。在最优培养条件下培养 24 h，菌株 ZLX10 的生物量是优化前的 1.98倍。【结论】通过优化条件可以明显提高菌株 ZLX10 的生物量，为应用于人工接种发酵雪茄烟叶提供基础。 |
| 英文摘要: |
| 【Objective】Microorganisms play a key role in the fermentation process of cigar tobacco leaves. In order to better improve the quality of cigar tobacco leaves, it is necessary to screen the dominant strains of cigars and apply them to the fermentation of cigar tobacco leaves. Therefore, it is necessary to optimize and expand the biomass of the dominant strains.【Method】16S sequencing and physiological and biochemical analysis were carried out on a dominant strain ZLX10 screened from cigars to identify the strain. At the same time, the composition of culture medium (carbon source, nitrogen source and inorganic salt) and culture conditions (inoculation amount, liquid content and seed age) were optimized through single factor and orthogonal test to improve its biomass.【Result】Based on 16S rDNA sequence comparison, the sequence homology between strain ZLX10 and Bacillus mojavensis (MT043920.1) reached 99.86%, and the strain had strong ability to degrade polysaccharide and protein macromolecule, which could be identified as Bacillus mojavenis. Through single factor and orthogonal test, the optimal medium formula of strain ZLX10 was obtained as follows: carbon source (sucrose) 50 g/L, nitrogen source (yeast extract) 20 g/L, inorganic salt (MgSO4) 0.25 g/L, and the optimal culture conditions were 1% (V/V) of inoculum, 30 mL/250 mL of liquid content, and 24 h of seed age. The biomass of strain ZLX10 was 1.98 times that before optimization when cultured under the optimal conditions for 24 h.【Conclusion】The biomass of strain ZLX10 can be significantly increased by optimizing the conditions, which provides a basis for artificial inoculation of fermented cigar tobacco leaves. |
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