| 吴 瑶 1,2，王再花 2，叶广英 2，杨 意 3，刘厚诚 4，周 荣 1.不同 LED 光质组合对两种观赏石斛组培及生长影响[J].广东农业科学,2023,50(2):32-41 |
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不同 LED 光质组合对两种观赏石斛组培及生长影响 |
| Effects of Different LEDs Light Combination on the Proliferation, Rooting and Growth of Two Kinds of Ornamental Dendrobium Tissue Culture Seedlings |
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| DOI：10.16768/j.issn.1004-874X.2023.02.004 |
| 中文关键词: LED 光质 ‘迷你’秋石斛 ‘口袋情人’春石斛 组织培养 种苗分级 |
| 英文关键词: LED light quality Dendrobium Mini Red Dendrobium Angel Baby ‘Love Pocket’ tissue culture grading of seedlings |
| 基金项目:国家重点研发计划项目（2020YFD1000400）；广东省重点领域研发计划项目（2019B020214005）；广州市科技计划项目 （202102021090）；广东省农业科技发展及资源环境保护管理项目（2022KJ131） |
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| 中文摘要: |
| 【目的】筛选适宜观赏性石斛克隆苗增殖及生根壮苗的优质 LED 光质组合，为种苗质量提升和标准化生产提供依据。【方法】设红、蓝、绿 3 种 LED 光和白光共 8 种不同光质组合，研究其对‘迷你’秋石斛、‘口袋情人’春石斛两种石斛组培苗增殖、生根壮苗及生长的影响。【结果】‘迷你’秋石斛在 R6B3G0 光质培养下增殖效果最好，增殖系数达到 2.35 个 / 株，其鲜重、干重和干物质含量分别为 3.941、0.2731 g/ 株和 7.06%；而在 R6B3G3 组合光质处理下生根效果最佳，根数和平均根长分别为 14.20 根 / 株和 2.81 cm。‘口袋情人’春石斛在 R3B6G0 光质培养下增殖效果最好，增殖系数达到 3.11 个 / 株，其鲜重、干重和干物质含量分别为 0.258、0.0218 g/ 株和 8.58%；在 R6B3G0 组合光质处理下生根效果最好，根数和平均根数分别为 10.98 根 / 株和 3.00 cm。采用PCA 主成分分析对上述两种石斛生根苗进行分级，‘迷你’秋石斛在 R3B3G3、R3B6G1、R3B6G3 光质培养下的一级苗最多、达 46.7~53.3%，驯化苗成活率达 100%；而‘口袋情人’春石斛在 R6B3G0 光质培养下的一级苗最多、达 53.3%，驯化苗成活率达 100%。【结论】适宜的红蓝绿 LED 光质组合对‘迷你’秋石斛和‘口袋情人’春石斛克隆苗的组培和生长有一定的促进作用，可培育更多优质的一级苗，研究结果为 LED 光源在观赏石斛组培生产中的应用及优质种苗的标准化生产提供技术参考。 |
| 英文摘要: |
| 【Objective】The study aims to screen the high-quality LEDs combination suitable for the proliferation, rooting and shoot strengthening of ornamental Dendrobium clonal seedlings, with a view to providing a basis for seedling quality improvement and standardized production.【Method】We studied the effects of 8 different light combinations including LEDs (red, blue and green) and white light (W) on the proliferation, rooting, shoot strengthening and growth of tissue culture seedlings of 2 popular Dendrobium varieties, Dendrobium Mini Red and Dendrobium Angel Baby ‘Love Pocket’.【Result】 D. Mini Red had the best proliferation effect in light quality culture of R6B3G0, the proliferation coefficient reached 2.35 per plant, and its fresh weight, dry weight and dry matter content were 3.941 g/plant, 0.2731 g/plant and 7.06%, respectively; the root number and average root length were 14.20 roots/plant and 2.81cm, respectively. The proliferation of D. Angel Baby ‘Love Pocket’ was the best in light quality culture of R6B3G0, the proliferation coefficient was 3.11 per plant, and the fresh weight, dry weight and dry matter content were 0.258 g/plant, 0.0218 g/plant and 8.58%, respectively; the best root effect was obtained under the light quality treatment of R6B3G0, and the root number and average root length were 10.98 roots/plant and 3.00 cm, respectively. Finally, PCA principal component analysis was used to classify the rooting seedlings of the above two Dendrobium varieties, and it was concluded that the first-class seedlings of D. Mini Red were the most in light quality culture of R3B3G3, R3B6G1 and R3B6G3 (46.7%-53.3%), and the survival rate of domesticated seedlings reached 100%; however, the first-class seedlings of D. Angel Baby ‘Love Pocket’ were the most in light quality culture of R6B3G0 (53.3%), and the survival rate of domesticated seedlings reached 100%.【Conclusion】Appropriate combination of red, blue and green LED light quality can promote the tissue culture and growth of clonal seedlings of D. Mini Red and D. Angel Baby ‘Love Pocket’, and cultivate more high-quality first-class seedlings. The results of this study provide technical reference for the application of LED light source in tissue culture production of ornamental Dendrobium and the standardized production of high-quality seedlings. |
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