菜心群体中 AUF2 基因的遗传变异及其与农艺性状的关联分析

黄依琳 1; 许玉富1; 李荣华 1; 李光光 2; 张 华 2; 郭培国 1; 夏岩石1

文章摘要

黄依琳 1，许玉富1，李荣华 1，李光光 2，张华 2，郭培国 1，夏岩石1.菜心群体中 AUF2 基因的遗传变异及其与农艺性状的关联分析[J].广东农业科学,2023,50(4):33-41

查看全文 HTML 菜心群体中 AUF2 基因的遗传变异及其与农艺性状的关联分析

Genetic Variations of AUF2 Gene and Their Association with Agronomic Traits in Flowering Chinese Cabbage

DOI：10.16768/j.issn.1004-874X.2023.04.004

中文关键词: 菜心 AUF2 基因多态性农艺性状关联分析优异等位位点

英文关键词: flowering Chinese cabbage AUF2 gene polymorphism agronomic trait association analysis elite allele

基金项目:广州市科技计划项目（202102010414）；广东省乡村振兴战略专项资金种业振兴项目（2022-NPY-03-001）

作者	单位
黄依琳 1，许玉富1，李荣华 1，李光光 2，张华 2，郭培国 1，夏岩石1	1. 广州大学生命科学学院作物抗逆国际合作研究中心，广东广州 510006；2. 广州市农业科学研究院，广东广州 510308

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中文摘要:

【目的】通过检测 Auxin Up-regulated F-box protein2（AUF2）基因在菜心群体中的自然变异，挖掘其优异等位基因，为菜心分子辅助育种提供理论参考。【方法】采用 PCR 扩增后直接测序的方法，检测AUF2 基因在 156 份菜心种质材料中的自然变异，利用 Tassel 5.0 软件的混合线性模型（MLM）对 AUF2 基因的变异位点与菜心群体的株高、单株质量、最大叶长、最大叶宽、最大叶柄长和叶绿素含量（SPAD）等 6 个农艺性状进行关联分析，以期发现显著影响菜心农艺性状的变异位点，并确定其优异等位变异及单倍型。【结果】经测序分析，在 AUF2 基因的 2 996 bp 扩增区域内共检测到 34 个变异位点，构成 36 个单倍型，表现出较高的核苷酸多样性（π = 0.00503）和单倍型多样性（Hd = 0.780），其上游非编码区 1 498 bp 内含 26 个 SNP 和 2 个InDel 位点，编码区 960 bp 内含 5 个 SNP 和 1 个 InDel 位点，而下游非编码区 538 bp 范围没有检测到变异位点。中性检验显示，AUF2 基因在群体中的 Tajima’s D 值、Fu and Li’s D* 值和 Fu and Li’s F* 值均为正值，且显著偏离中性选择，可能是群体平衡选择的结果。经关联分析发现，4 个 SNP 位点与菜心的单株质量、最大叶长、最大叶宽和最大叶柄长等 4 个农艺性状呈显著相关性，其表型解释率在 5.04% ~ 6.52% 范围；4 个优异等位变异构成的单倍型 Hap2 能显著增加菜心的单株质量，极显著地提高最大叶柄长的长度。【结论】AUF2 基因在菜心群体中存在丰富的变异，对菜心的部分农艺性状有显著影响，其优异等位变异和单倍型将有利于菜心品种的遗传改良。

英文摘要:

【Objective】By detecting the natural variation of Auxin Up-regulated F-box protein2 (AUF2) gene and exploring the elite alleles associated with agronomic traits in order to provide theoretical references for molecular marker assisted breeding of flowering Chinese cabbage.【Method】PCR amplification and sequencing was used to detect the natural variations in AUF2 gene among 156 flowering Chinese cabbage accessions. By using the mixed linear model (MLM) of Tassel 5.0 software, the correlation between the variation sites of AUF2 gene and six agronomic traits of flowering Chinese cabbage was analyzed, including plant height, single plant weight, maximum leaf length, maximum leaf width, maximum petiole length and chlorophyll content (SPAD), and the polymorphic sites significantly associated with agronomic traits would be identified. Then, elite alleles and excellent haplotypes were determined.【Result】Through sequencing analysis, a total of 34 mutation sites were detected in the 2 996 bp amplification region of AUF2 gene, including 36 haplotypes. The AUF2 gene showed high nucleotide diversity (π = 0.00503) and haplotype diversity (Hd = 0.780). Among them, 2 InDels and 26 SNPs were in the 1 498 bp upstream non-conding region, and 1 InDel and 5 SNPs were in the 960 bp coding region. No mutation sites were detected in the 538 bp downstream non-conding region. Neutral test analysis showed that Tajima’s D value, Fu and Li’s D* value, and Fu and Li’s F* value of the AUF2 gene were positive, and deviated significantly from neutral evolutionary model. One plausible reason was that flowering Chinese cabbage population was subject to balancing selection. The association analysis showed that 4 SNPs were significantly associated with at least one of the four agronomic traits, including single plant weight, maximum leaf length, maximum leaf width and maximum petiole length. The phenotypic variation of a given trait explained by each associated SNP ranged from 5.04% to 6.52%. The haplotype Hap2, which contained elite alletes of the four SNPs mentioned above, could significantly raise the single plant weight, and remarkably increase the length of the maximum petiole.【Conclusion】There are abundant variations in the AUF2 gene, which has a significant impact on the agronomic traits of flowering Chinese cabbage. These elite alleles and excellent haplotypes of AUF2 gene will benefit the genetic improvement of flowering Chinese cabbage.

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