副猪格拉菌血清 2 型毒力及耐药性分析

王治方; 徐引弟; 朱文豪; 焦文强; 张青娴; 李海利; 许 峰; 王克领

文章摘要

王治方，徐引弟，朱文豪，焦文强，张青娴，李海利，许峰，王克领.副猪格拉菌血清 2 型毒力及耐药性分析[J].广东农业科学,2023,50(5):112-120

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Analysis of Virulence and Drug Resistance of Glaesserella parasuis Serotype 2

DOI：10.16768/j.issn.1004-874X.2023.05.013

中文关键词: 副猪格拉菌血清 2 型毒力基因致病性药敏试验耐药基因

英文关键词: Glaesserella parasuis serotype 2 virulence gene pathogenicity drug sensitivity test drug-resistant gene

基金项目:国家重点研发计划项目（2021YFD1301205）；河南省科技攻关项目（212102110378）；河南省农业科学院自主创新项目（2023ZC055）

作者	单位
王治方，徐引弟，朱文豪，焦文强，张青娴，李海利，许峰，王克领	河南省农业科学院畜牧兽医研究所 / 河南省畜禽繁育与营养调控重点实验室，河南郑州 450002

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中文摘要:

【目的】副猪格拉菌是引起猪格拉泽氏病的病原，是危害养猪业最为严重的细菌性病原之一，该菌血清型众多，不同血清型之间交叉保护力弱。血清 2 型是中等毒力毒株，近年来临床分离比例越来越高，危害越来越严重。从河南某规模化猪场保育猪群分离到副猪格拉菌血清 2 型并进行系列试验，为科学防控该病提供参考。【方法】对副猪格拉菌临床疑似病例猪只无菌采集肺脏、气管、心血等样品，通过细菌分离纯化、革兰氏染色镜检、常规 PCR、豚鼠致病性试验及琼脂扩散药敏试验等方法，对疑似细菌进行形态观察、鉴定、血清型分型、毒力基因检测、耐药基因检测、致病性和耐药性等一系列生物学特性研究。【结果】从肺脏样品中分离获得 1 株副猪格拉菌，经鉴定为血清 2 型，该菌株携带 vta1、vta2、vta3、wza、ompP2、nanH、cdtA、cdtB、cdtC、espP2 毒力基因，对豚鼠有较强的致病力。该菌株携带有 aadA1、strA、strB、aphA1、tet(B)、sul2 多个耐药基因，对青霉素 G、卡那霉素、阿米卡星、链霉素、四环素、土霉素、复方新诺明有较强的耐药性；对头孢噻呋、头孢他啶、阿莫西林、氨苄西林、左氟沙星、氧氟沙星、恩诺沙星、环丙沙星敏感性较好，临床用药效果显著。【结论】分离出的副猪格拉菌 2 型菌株毒力较强，且能透过血脑屏障，有明显的多重耐药性。研究结果可为副猪格拉菌血清 2 型流行病学调查、致病机制研究以及临床防控措施制定提供参考。

英文摘要:

【Objective】Glaesserella parasuis (GPS) is the pathogen that causes Glässer’s disease in pigs. It is one of the most serious bacterial pathogens that harm pig industry. There are many serotypes of this bacterium, and the crossprotection between different serotypes is weak. Serotype 2 is a moderately virulent strain, and the proportion of clinical isolates has been increasing, resulting in increasingly serious harm in recent years. GPS serotype 2 was isolated from nursery pigs in a large-scale pig farm in Henan Province and a series of tests were conducted, and provided reference for scientific prevention and control of the disease. 【Method】In this study, lung, trachea, blood and other samples of GPS clinically suspected cases pigs were collected. Bacterial isolation and purification, Gram staining microscopy, routine PCR, guinea pig pathogenicity test, AGAR diffusion drug sensitivity test and other methods were used. Morphological observation, identification, serotyping, virulence gene detection, drug-resistant gene detection, pathogenicity and drug resistance of suspected bacteria were studied. 【Result】A strain of GPS was isolated from lung samples and identified as serotype 2. The strain carried virulence genes of vta1, vta2, vta3, wza, ompP2, nanH, cdtA, cdtB, cdtC and espP2, and showed strong virulence to guinea pigs. The strain carried drug-resistant genes aadA1, strA, strB, aphA1, tet(B), sul2, and had strong resistance to penicillin G, kanamycin, amicacin, streptomycin, tetracycline, oxytetracycin, and cotrimoxazole. It had good sensitivity to ceftiofurme, ceftazidime, amoxicillin, ampicillin, levfloxacin, ofloxacin, ennofloxacin and ciprofloxacin, and had significant clinical effect. 【Conclusion】The isolates of GPS serotype 2 have strong virulence, can pass through the blood-brain barrier, and have obvious multi-drug resistance. The results of this study provide reference for the epidemiological investigation of GPS serotype 2, pathogenic mechanism, and the formulation of clinical prevention and control measures.

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