| 朱云娜，杨景辉，王 斌，王玉昆，周裕荣，卢威宇，刘建国.菜心 BcCIPK23 基因克隆及表达特性分析[J].广东农业科学,2023,50(9):89-98 |
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菜心 BcCIPK23 基因克隆及表达特性分析 |
| Cloning and Expression Characteristics Analysis of BcCIPK23 in Flowering Chinese Cabbage |
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| DOI：10.16768/j.issn.1004-874X.2023.09.009 |
| 中文关键词: 菜心 BcCIPK23 基因克隆 生物信息学分析 表达特性 |
| 英文关键词: flowering Chinese cabbage BcCIPK23 gene cloning bioinformatics analysis expression characteristics |
| 基金项目:国家自然科学基金（32202465）；广东省自然科学基金（2023A1515012687）；韶关学院博士科研启动项目（99000613） |
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| 中文摘要: |
| 【目的】CIPK23 基 因 在 植 物 生 长 发 育 和 矿 质 营 养 吸 收 中 具 有 重 要 作 用， 但 在 菜 心 中 关 于BcCIPK23 基因及其功能的研究尚无报道，该研究可为探讨 BcCIPK23 在菜心植株生长发育和氮代谢中的生物学功能提供理论参考。【方法】以‘油绿 501’菜心为材料，采用同源克隆法克隆到菜心 BcCIPK23 全长序列，对其编码蛋白进行生物信息学分析，探究其组织表达特性和对不同氮源的响应。【结果】菜心 BcCIPK23 基因 CDS 全长为 1 450 bp，编码 482 个氨基酸，分子量是 53.41 kD，理论等电点为 9.28，具有 CIPK 家族成员的STKc-SnRK3 和 CIPK-C 保守结构域。系统进化树分析表明，菜心 BcCIPK23 和白菜 BrCIPK23 进化关系最近。BcCIPK23 在菜心叶片、叶柄、薹茎、根系、花等组织中均有表达，但主要集中在叶片、叶柄、薹茎中表达；BcCIPK23 表达也受氮源及其水平的影响，无论供 NO3- 还是供 NH4+，其表达水平均随氮浓度增加而降低。推测该基因在叶片、薹茎发育和氮素吸收利用中发挥关键作用。【结论】菜心 BcCIPK23 为 CIPK 基因家族成员，主要在叶片等生长活跃的器官高表达，且可响应氮源及氮浓度变化，参与植物生长发育和氮代谢过程。 |
| 英文摘要: |
| 【Objective】CIPK23 plays an important role in the development and mineral absorption in many plants, however, there are no relevant reports on CIPK23 gene and its function in flowering Chinese cabbage. The research will provide some theoretical references for further study of the function of BcCIPK23 in the growth and development and nitrogen metabolism in flowering Chinese cabbage.【Method】The full-length cDNA sequence of BcCIPK23 was obtained by homologous cloning method from flowering Chinese cabbage variety ‘Youlv 501’. The encoded BcCIPK23 protein were analyzed by bioinformatics, and the expression characteristics of BcCIPK23 in different tissues and its response to different nitrogen sources of flowering Chinese cabbage were analyzed. 【Result】The CDS full length of BcCIPK23 gene in flowering Chinese cabbage was 1 450 bp, encoding 482 amino acids, with a molecular weight of 53.41 kD, and a theoretical isoelectric point of 9.28. The BcCIPK23 sequence contained the domains of STKc-SnRK3 and CIPK-C, which were the conserved domains of CIPK family. The phylogenetic analysis showed that BcCIPK23 from flowering Chinese cabbage was antigenically closely related to BrCIPK23 from Brassica rapa. The qRT-PCR results showed that BcCIPK23 was expressed in leaves,petioles, stalks, roots, flowers and other tissues of flowering Chinese cabbage, but was mainly expressed in leaves, petioles and stalks. The expression level of BcCIPK23 was significantly affected by different sources and concentrations of nitrogen, decreased significantly with the increase of NO3- or NH4+ concentration. It was predicted that BcCIPK23 may play a key role in the growth and development of leaves and stalks, and the absorption and utilization of nitrogen in flowering Chinese cabbage.【Conclusion】BcCIPK23 is a member of the CIPK gene family, which is highly expressed in leaves and other active organs.It can respond to changes in nitrogen sources and nitrogen concentrations, and is involved in plant growth and development and nitrogen metabolic processes. |
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