| 向星星，丰 锋，干雨露，于奕宁.耐盐闽粤石楠组培快繁体系的建立[J].广东农业科学,2023,50(9):191-197 |
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耐盐闽粤石楠组培快繁体系的建立 |
| Establishment of Tissue Culture and Rapid Propagation System for Salt-tolerant Photinia benthamiana Hance |
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| DOI：10.16768/j.issn.1004-874X.2023.09.020 |
| 中文关键词: 闽粤石楠 耐盐 茎段培养 诱导 增殖 生根 |
| 英文关键词: Photinia benthamiana Hance salt-tolerant stem segment culture induction proliferation rooting |
| 基金项目:国家重点研发计划项目（2017YFD0202102）；云南省科技人才与平台计划项目“云南省丰锋专家工作站”（202305AF150146） |
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| 中文摘要: |
| 【目的】建立耐盐闽粤石楠组培快繁技术体系。【方法】以耐盐闽粤石楠实生苗茎段为试验材料，采用正交试验设计和方差分析，探讨外植体最佳灭菌处理、腋芽诱导、不定芽增殖和生根培养的最佳培养基。【结果】耐盐闽粤石楠带芽茎段的最佳灭菌方法为洗衣粉漂洗 20 min、流水冲洗 60 min、75%。乙醇灭菌 10 s、0.15%氯化汞灭菌 3 min，无菌水清洗 5 次，外植体成活率最高、为 76.67%。最佳诱导培养基为 MS + 1.0 mg/L 6-BA+0.2 mg/L NAA+30 g/L 蔗糖 + 5.5 g/L 琼脂，有利于腋芽诱导，此培养条件下诱导率为 73.33%，平均萌芽数 1.03 个，显著高于其他处理，且不定芽叶绿色，长势较好；最佳增殖培养基为 1/2MS+2.0 mg/L 6-BA+0.5 mg/L NAA+ 30 g/L 蔗糖 + 5.5 g/L 琼脂，有利于不定芽增殖，此培养条件下增殖系数达 3.1，显著高于其他处理，且不定芽分化多，叶色绿，长势良好；最佳生根培养基为 1/2MS+0.4 mg/L IBA+ 30 g/L 蔗糖 + 5.5 g/L 琼脂，有利于不定芽生根，此条件下培养 60 d 生根率为 87%，平均根数 6.22 条，平均根长 3.78 cm，显著高于其他处理，且根系健壮。【结论】初步建立耐盐闽粤石楠组培快繁体系，为沿海滩涂利用提供种苗支持。 |
| 英文摘要: |
| 【Objective】The study aims to establish a tissue culture and rapid propagation system for salt-tolerant Photinia benthamiana Hance.【Method】The stem segments of salt-tolerant Photinia benthamiana Hance seedlings were used as experimental materials. The orthogonal test design and variance analysis were used to explore the best sterilization treatment of explants, axillary bud induction, adventitious bud proliferation and optimal medium for rooting culture.【Result】The best sterilization method of salt-tolerant Photinia benthamiana Hance stems with buds was as follows: rinsing with washing powder for 20 minutes, washing with running water for 60 minutes, then sterilizi ng with 75% ethanol for 10 seconds and with 0.15% mercuric chloride for 3 minutes, and washing with sterile water for 5 times. The survival rate of explants was the highest, which was 76.67%. The optimal induction medium MS+1.0 mg/L 6-BA+0.2 mg/L NAA+30 g/L sucrose+5.5 g/L agar was conducive to axillary bud induction. Under this culture condition, the induction rate was 73.33%, the average number of buds was 1.03, which was significantly higher than that of other treatments, and the leaves of adventitious buds were green and grew well. The proliferation medium 1/2MS+2.0 mg/L 6-BA+0.5 mg/L NAA+30 g/L sucrose+5.5 g/L agar was beneficial to the proliferation of adventitious buds. Under this culture condition, the proliferation coefficient was 3.1, which was significantly higher than that of other treatments, and the adventitious buds had more differentiation, with green leaves and good growth. The rooting medium 1/2MS+0.4 mg/L IBA+30 g/L sucrose+5.5 g/L agar was beneficial to the rooting of adventitious buds. Under this culture condition, the rooting rate was 87%, the average number of roots was 6.22, and the average root length was 3.78 cm, significantly higher than that of other treatments, and the roots were robust.【Conclusion】In the study, the tissue culture and rapid propagation system for salt-tolerant Photinia benthamiana Hance is preliminarily established, which provides seedling support for the utilization of coastal tidal flats. |
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