| 张婷婷 1，谭永强 1，刘 莹 1，袁改改 1 ，芮荣涛 1，周 扬 2，凌 冬 1.盐胁迫下转 DcCIPK24 拟南芥的转录组比较分析[J].广东农业科学,2023,50(10):75-84 |
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盐胁迫下转 DcCIPK24 拟南芥的转录组比较分析 |
| Comparative Transcriptome Analysis of DcCIPK24 Transgenic Arabidopsis Under Salt Stress |
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| DOI：10.16768/j.issn.1004-874X.2023.10.009 |
| 中文关键词: 铁皮石斛 DcCIPK24 转基因 差异基因 代谢通路 |
| 英文关键词: Dendrobium catenatum DcCIPK24 transgenic differential genes metabolic pathway |
| 基金项目:海南省自然科学基金（319MS009）；海南省教育厅项目（Hys2020-242）；海南大学横向课题（HDKYH-2023093）；襄阳市农业科学院特色产业科普基地项目（XAAS2022025） |
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| 中文摘要: |
| 【 目 的】 探 究 铁 皮 石 斛（Dendrobium catenatum Lindl.）DcCIPK24 的 下 游 响 应 基 因， 为 研 究DcCIPK24 提高耐盐性的分子调控途径提供指导。【方法】利用 Illumina NovaSeq 6000 测序平台对 200 mmol/L NaCl 处理和对照组的转 DcCIPK24 拟南芥、野生型拟南芥进行测序，分析差异表达基因的富集通路，并对通路中的差异基因进行荧光定量 PCR 验证。【结果】盐胁迫下，从 2 个转基因拟南芥株系与野生型拟南芥的比较组合中共鉴定出 96 个差异表达基因；GO 注释分析发现 96 个差异基因主要被富集在分子功能相关通路；KEGG 富集分析发现差异基因主要被注释在氨基糖和核苷酸糖代谢、植物 MAPK 信号通路和甘油酯代谢通路中，选取上调表达的差异基因 AtGPAT5、AtSIRK、AtMEE25、AtUGE5 和下调表达基因 AtAMT1-2、AtATTI3、AtCYP71A25、AtLHCB4.3 进行实时荧光定量 PCR 验证，结果表明盐胁迫下 8 个差异基因表达趋势与转录组数据基本一致。【结论】盐胁迫下，DcCIPK24 主要通过氨基糖和核苷酸糖代谢途径、植物 MAPK 信号途径和甘油酯代谢通路响应耐盐。 |
| 英文摘要: |
| 【Objective】This study aimed to explore the downstream response genes of Dendrobium catenatum Lindl. DcCIPK24, and provided guidance for studying the molecular regulatory pathway of DcCIPK24 to improve salt tolerance. 【Method】The DcCIPK24 transgenic Arabidopsis thaliana and wild type plants under 200 mmol/L NaCl treatment and normal conditions were used for RNA-seq on the illumina NovaSeq 6000 sequencing platform. The enrichment pathway of differentially expressed genes (DEGs) was analyzed, and their expression patterns were verified by quantitative real-time PCR. 【Result】A total of 96 DEGs were identified from transgenic A. thaliana and wild type plants under salt stress. GO annotation analysis showed that 96 DEGs were mainly enriched in molecular function pathways. KEGG enrichment analysis found that DEGs enriched in amino sugar and nucleotide sugar metabolism, plant MAPK signaling pathway and glycerolipid metabolism. The DEGs including four upregulated genes (AtGPAT5, AtSIRK, AtMEE25, and AtUGE5) and four downregulated genes (AtAMT1-2, AtATTI3, AtCYP71A25, and AtLHCB4.3) were selected for qRT-PCR validation. The results showed that the trends of 8 DEGs’ expression were basically consistent with the RNA-seq data. 【Conclusion】DcCIPK24 responded to salt tolerance mainly through amino sugar and nucleotide sugar metabolism, plant MAPK signaling pathway, and glycerolipid metabolism. |
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