| 谭嘉娜，官锦燕，许玉婵，陈双艳，罗剑飘，罗青文.重瓣红纸扇组培苗工厂化繁育技术体系构建[J].广东农业科学,2023,50(10):130-140 |
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重瓣红纸扇组培苗工厂化繁育技术体系构建 |
| Construction of Factory Breeding Technology System for Tissue Cultured Seedlings of Mussaenda hybird |
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| DOI：10.16768/j.issn.1004-874X.2023.10.014 |
| 中文关键词: 重瓣红纸扇 组培苗 光质 高效 工厂化繁育技术 |
| 英文关键词: Mussaenda hybird tissue cultured seedlings light quality high efficiency factory breeding technology |
| 基金项目:湛江市科技计划项目“RCEP 科技交流合作专项”（2022A01035）；湛江市科技计划项目（2021A05012） |
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| 摘要点击次数: 601 |
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| 中文摘要: |
| 【目的】建立重瓣红纸扇组培苗高效的工厂化繁育技术体系，为实现该品种规模化生产提供技术支撑。【方法】以重瓣红纸扇母株的茎尖、茎段为外植体，研究消毒剂种类与方式、基本培养基、激素种类及配比、光质等因素对其组培苗繁育效率的影响，并研究不同季节对组培苗移栽成活率及生长的影响。【结果】重瓣红纸扇外植体遇酒精容易死亡，无菌水清洗可以减轻酒精对外植体的毒害，10%H2O2 处理 10 min 与0.1% HgCl2 处理 8 min 可获得大量的无菌材料；3/2MS 基本培养基诱导芽体长势好；外植体在 3/2MS+BA 3.0mg/L+NAA 0.2 mg/L 培养基上芽的诱导率高且芽体粗壮；探讨不同配方下的增殖方式对组培苗工厂化生产效率的影响，结果发现 3/2MS+BA 0.5 mg/L+NAA 0.1 mg/L+ 蛋白胨 0.1 g/L 培养基上以切段繁殖方式效率更高，组培苗在3/2MS+NAA 0.04~0.05 mg/L+IBA 0.01 mg/L 培养基上生根率达 100%，根系粗壮，植株生长旺盛；不同季节对重瓣红纸扇组培苗的驯化移栽影响差异显著；不同光质对其组培苗的生长发育阶段和叶绿素含量均存在显著性差异，荧光灯处理适用于增殖扩繁，LED 灯 80% 红光 +20% 蓝光处理适用于生根阶段壮苗培养。【结论】试验结果获得重瓣红纸扇外植体有效的消毒方法、组培芽体诱导增殖和生根壮苗适宜的培养基配方与光质条件，为重瓣红纸扇工厂化育苗提供了理论依据。 |
| 英文摘要: |
| 【Objective】An efficient factory breeding technology system for tissue cultured seedlings of Mussaenda hybird was established to provide technical support for the large-scale production of the variety.【Method】The stem tips and stem segments of the mother plant of M. hybird were used as explants to study the effects of different disinfection types and treatments, basic medium types and hormone ratios and light quality on the reproductive efficiency of their seedlings. And the effects of different seasons on the survival rate and growth of transplanted seedlings were explored.【Result】Explants of M. hybird were easy to die when exposed to alcohol, and sterile water washing could reduce the toxicity of alcohol to explants; 10% H2O2 treatment for 10 min and 0.1% HgCl2 treatment for 8 min could obtain a large number of sterile materials. Explants had high bud induction rate and strong buds when cultured on 3/2MS + 6-BA 3.0 mg/L + NAA 0.2 mg/L. According to the results of the effects of proliferation methods under different formulations on factory production efficiency of tissue cultured seedlings, propagation of M. hybird was more efficient on 3/2MS + 6-BA 0.5 mg/L + NAA 0.1 mg/L + peptone
0.1 g/L by stems; the rooting rate of tissue cultured seedlings was 100% on 3/2 MS + NAA 0.04-0.05 mg/L + IBA 0.01 mg/L, the roots were strong, and the plants grew well. There were significant differences in the effects of different seasons on the domestication and transplanting of tissue cultured seedlings of M. hybird. It was found that significant differences in the growth and development and chlorophyll content of tissue culture seedlings with different light qualities. Fluorescent lamp treatment was suitable for proliferation and propagation, and LED with 80% red light +20% blue light was suitable for strong seedlings cultivation at rooting stage.【Conclusion】Effective disinfection method of explants, and suitable medium formula and light quality conditions for the induction and proliferation of buds were obtained, which provided a theoretical basis for the factory breeding of Mussaenda hybird. |
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