| 蔡 欣，黄钰杏，崔明月，林明丽，王一婷，卢 月，王丽敏.水稻 OsbHLH109 基因的表达分析[J].广东农业科学,2023,50(12):120-130 |
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水稻 OsbHLH109 基因的表达分析 |
| Expression Analysis of OsbHLH109 Gene in Rice |
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| DOI：10.16768/j.issn.1004-874X.2023.12.012 |
| 中文关键词: 水稻；bHLH 转录因子；OsbHLH109 表达分析；非生物胁迫 |
| 英文关键词: rice bHLH transcription factor OsbHLH109 expression analysis abiotic stress |
| 基金项目:国家自然科学基金（32101647）；仲恺农业工程学院仲华基因产业学院项目（ZHCY2023007） |
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| 中文摘要: |
| 【目的】bHLH 转录因子家族是真核生物中重要的转录因子家族，在植物生长发育、次生代谢和逆境应答中发挥重要作用。分析水稻（Oryza sativa L.）bHLH 转录因子基因 OsbHLH109（LOC_Os01g67480）对逆境胁迫和激素的响应模式，为进一步研究 OsbHLH109 在逆境胁迫和激素响应过程中的功能提供理论依据。【方法】对 OsbHLH109 进行生物信息学分析，同时利用定量 PCR（qRT-PCR）技术分析 OsbHLH109 在水稻品种中花 11 不同组织、不同激素和非生物胁迫处理下的表达模式。【结果】OsbHLH109 编码区全长为 1 164 bp，包含 6 个外显子，编码 388 个氨基酸，是一个含有 HLH 保守结构域的 bHLH 转录因子。系统进化分析显示，OsbHLH109 与玉米等单子叶植物中的同源蛋白亲缘关系较近，与双子叶植物中的同源蛋白亲缘关系相对较远。顺式作用元件分析表明，OsbHLH109 的启动子区含有 24 个植物激素响应元件和 25 个环境胁迫响应元件。qRT-PCR 分析表明，OsbHLH109 在水稻根、茎、叶和幼穗等组织中均有表达，但在叶片中的表达量最高；激素和非生物胁迫处理结果表明，叶片中 OsbHLH109 对细胞分裂素（6-Benzylaminopurine, 6-BA）、生长素（Indole acetic acid, IAA）、赤霉素（Gibberellic acid, GA3）、脱落酸（Abscisic acid, ABA）等激素处理的响应表达均达到显著差异水平；且叶片中 OsbHLH109 在低温、干旱、高温、盐胁迫等逆境处理的响应表达均达到显著差异水平，表明 OsbHLH109 的表达受 6-BA、IAA、GA3、ABA、高温、低温、PEG6000 和 NaCl 的诱导，推测其在水稻非生物胁迫响应过程中具有重要作用。【结论】水稻 OsbHLH109 为 bHLH 转录因子家族成员，主要在叶片中高表达，OsbHLH109 基因的表达受高温、低温、盐害等外界因素调控，同时响应 6-BA、IAA、GA3、ABA 等激素信号，推测其可能通过 ABA 等激素信号转导途径参与水稻的非生物胁迫响应。 |
| 英文摘要: |
| 【Objective】The bHLH transcription factor family is an important family of transcription factors in eukaryotes, which plays an important role in plant growth and development, secondary metabolism and stress response. Analysis of the response pattern of bHLH transcription factor gene OsbHLH109 (LOC_Os01g67480) to stress and hormones in rice (Oryza sativa L.) could provide a theoretical basis for further researches on the functions of OsbHLH109 in stress and hormone responses.【Method】The bioinformatics of OsbHLH109 was analyzed, and the expression patterns of OsbHLH109 in different tissues of Rice Zhonghua 11 under different hormone and abiotic stresses were analyzed by quantitative PCR (qRT-PCR).【Result】The encoding region of OsbHLH109 was 1 164 bp in length, containing 6 exons and encoding 388 amino acids, which was a bHLH transcription factor containing a conserved domain of HLH. Phylogenetic analysis showed that OsbHLH109 was closely related to the homologous proteins in monocots and Dicotyledones. The analysis of cis-acting elements showed that the promoter region of OsbHLH109 contained 24 plant hormone response cis-elements and 25 environmental stress response cis-elements. The qRT-PCR analysis showed that OsbHLH109 was expressed in roots, stems, leaves and young panicles of rice, but the expression level was the highest in leaves. The results of hormone treatment response expression profile analysis showed that the response expression differences of OsbHLH109 in leaves to 6-Benzylaminopurine (6-BA), indole acetic acid (IAA), gibberellic acid (GA3) and abscisic acid (ABA) treatments all reached significant levels. The results of stress treatment response expression profile analysis showed that the response of OsbHLH109 to low temperature stress, drought stress, high temperature stress and salt stress were significantly different in leaves, indicating that the expression of OsbHLH109 was induced by 6-BA, GA3, IAA, ABA, high temperature, low temperature, PEG6000 and NaCl, which suggested that OsbHLH109 was essential for the abiotic stress responses in rice.【Conclusion】Rice OsbHLH109 is a member of the bHLH transcription factor family and is highly expressed in leaves. The expression of OsbHLH109 gene is regulated by high temperature, low temperature and salt stress, and responds to hormone signals such as 6-BA, IAA, GA3 and ABA. It is predicted that it may be involved in abiotic stress response of rice through ABA and other hormone signal transduction pathways. |
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