| 敖广宇，陈庚华，夏燚琳，罗 文.高脂饲喂对肉鸡腹脂和肝脏组织RNA 可变剪接的影响[J].广东农业科学,2024,51(2):112-123 |
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高脂饲喂对肉鸡腹脂和肝脏组织RNA 可变剪接的影响 |
| Effects of High-fat Diet on Alternative Splicing of RNA in Abdominal Fat and Liver Tissues of Broilers |
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| DOI：10.16768/j.issn.1004-874X.2024.02.011 |
| 中文关键词: 杏花鸡 高脂饲料 腹脂沉积 转录组 可变剪接 差异表达基因 |
| 英文关键词: Xinghua broiler high-fat feed abdominal fat deposition transcriptome alternative splicing differentially expressed gene |
| 基金项目:国家自然科学基金（3227286，31972544） |
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| 摘要点击次数: 561 |
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| 中文摘要: |
| 【目的】腹脂过度沉积会降低肉鸡抗病力和屠宰率。RNA 可变剪接是影响基因功能的重要调控方式，对肉鸡腹脂沉积过程的 RNA 可变剪接事件进行系统分析，有助于进一步了解肉鸡腹脂沉积的分子调控机理。【方法】利用高脂饲喂肉鸡和普通饲喂肉鸡肝脏组织和腹脂组织的转录组测序结果，鉴定高脂饲喂过程中肉鸡腹脂和肝脏组织的差异表达和差异剪接基因。采用 GO 功能注释、KEGG 通路富集分析和 Metascape 富集分析对显著差异剪接基因进行分析，并对显著差异剪接事件进行可视化处理，以及分析调控这些差异剪接事件的剪接因子。【结果】对高脂饲喂肉鸡与普通饲喂肉鸡的腹脂和肝脏组织进行转录组测序，在腹脂组织中发现 233 个显著差异表达基因和349 个显著差异剪接基因，对显著差异剪接基因进行富集分析，发现显著差异剪接基因主要富集于细胞分裂、细胞生长等细胞增殖相关生物学进程，还富集于甘油磷脂代谢和胰岛素信号通路等脂肪生成相关通路。在肝脏组织中发现 276 个显著差异表达基因和 224 个显著差异剪接基因，对显著差异剪接基因进行富集分析，发现显著差异剪接基因主要富集于蛋白质乙酰化、蛋白质内部氨基酸乙酰化和 mRNA 加工等细胞代谢相关生物进程，还富集于胰岛素信号通路、胰高血糖素信号通路、AMPK 信号通路等脂肪生成相关通路。在腹脂和肝脏的显著差异剪接基因中，共筛选到影响肉鸡腹脂沉积性状的相关候选基因 4 个，包括 GPAT4、RELCH、CYP39A1 和 PPARA。【结论】研究结果初步揭示高脂饲喂下肉鸡腹脂和肝脏组织的 RNA 可变剪接调控规律。推断高脂饲喂可能通过影响基因可变剪接过程，进而激活前脂肪细胞的增殖和脂肪生成相关细胞代谢，从而导致肉鸡的腹脂沉积现象；剪接因子对靶基因的调控显著参与影响肉鸡的腹脂沉积过程。 |
| 英文摘要: |
| 【Objective】Excessive deposition of abdominal fat reduces disease resistance and slaughter rate of broilers. RNA alternative splicing is an important regulatory modality that affects gene function. Systematic analysis of RNA alternative splicing events during broiler abdominal fat deposition contributes to further understanding of the molecular regulation mechanism of broiler abdominal fat deposition.【Method】The transcriptome sequencing results of liver and abdominal fat tissues in high-fat-fed and normal-fed broilers were used to identify the differentially expressed and alternative spliced genes in abdominal fat and liver tissues during high-fat feeding. Significantly differentially spliced genes were analyzed by GO functional annotation, KEGG pathway enrichment analysis and Metascape enrichment analysis, and visualization processing was conducted for the significantly differential alternative splicing events, then splicing factors regulating these differential splicing
events were analyzed.【Result】Transcriptome sequencing was performed on the abdominal fat and liver tissues of high-fat-fed broilers and normal-fed broilers. 233 significantly differentially expressed genes and 349 significantly differentially alternative spliced genes were found in the abdominal fat tissues. It was found that the significantly differential spliced genes were mainly enriched in cell proliferation, such as cell division and cell growth, as well as in pathways related to adipogenesis, such as glycerophospholipid metabolism and insulin signaling pathway. In liver tissues, 276 significantly differentially expressed genes and 224 significantly differential spliced genes were found, and the enrichment analysis of the significantly differential spliced genes revealed that these genes were mainly enriched in biological processes related to cellular metabolism such as protein acetylation, intra-protein amino acid acetylation and mRNA processing and also enriched in adipogenesis-related pathways such as insulin signaling pathway, glucagon signaling pathway and AMPK signaling pathway. Among the significantly differential spliced genes in abdominal fat and liver, a total of four candidate genes related to influencing abdominal fat deposition traits in broiler were screened, including GPAT4, RELCH, CYP39A1, and PPARA.【Conclusion】The results preliminarily revealed the patterns of RNA alternative splicing regulation in abdominal fat and liver tissues of broilers under high-fat feeding. It was inferred that high-fat feeding may lead to abdominal fat deposition in broilers by affecting the alternative splicing process of genes, which may activate the proliferation of preadipocytes and the metabolism of adipogenesis-related cells. Regulation of target genes by splicing factors was significantly involved in influencing the process of abdominal fat deposition in broilers. |
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