| 范思庆，谢钊启，杨佳欣，郭 敏，程春松.白木香 ARF 基因家族的鉴定及结香表达分析[J].广东农业科学,2024,51(5):44-55 |
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白木香 ARF 基因家族的鉴定及结香表达分析 |
| Identification of ARF Gene Family in Aquilaria sinensis (Lour.) Gilg and Expression Analysis of Aagrwood-induced |
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| DOI：10.16768/j.issn.1004-874X.2024.05.004 |
| 中文关键词: 生长素响应因子 白木香 AsARF 基因家族 RNA-seq 分析 结香机制 |
| 英文关键词: auxin response factor Aquilaria sinensis AsARF gene family RNA-seq analysis aroma formation
mechanism |
| 基金项目:江西省双千人才 - 自然科学领军人才长期项目（jxsq2023101038）；江西省急需紧缺海外人才项目
（20223BCJ25027）；九江市基地和人才计划重点领域科技创新团队项目（S2022TDJS029） |
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| 中文摘要: |
| 【目的】 生长素响应因子（Auxin response factor, ARF）是生长素信号转导中的重要转录因子，参与
植物花果发育、器官形态形成、激素调节和应激反应等生理生化过程。旨在探究白木香〔Aquilaria sinensis (Lour.)
Gilg〕ARF 基因（AsARF）在结香过程中的生物学功能。【方法】利用生物信息学方法对 AsARF 进行全基因组鉴定
和分析，通过转录组数据分析白木香受到机械损伤时 ARF 基因家族成员的表达模式。【结果】从全基因组水平鉴
定了 21 个 AsARF 基因，生物信息学分析结果显示，21 个 AsARF 基因分为 5 个类群，同一类群的 AsARF 基因具有
相似的基因结构和蛋白保守基序，而不同类群的 AsARF 基因在外显子和内含子的数量上存在显著差异。此外，顺
式作用元件分析发现 AsARF 基因启动子区域含有大量与光、厌氧和赤霉素等响应元件有关的序列。基因复制和共
线性分析结果表明，片段复制事件对于白木香 ARF 基因家族的进化和扩张起着重要作用。与水稻 ARF 基因相比，
白木香和拟南芥的 ARF 基因更为密切相关。此外，转录组数据分析表明，白木香在受到机械损伤时，AsARF5、
AsARF6、AsARF19、AsARF20 和 AsARF21 的表达量明显下降。【结论】首次对白木香 ARF 基因家族进行全基因组
鉴定，并对其理化性质、系统进化、基因结构、保守基序、启动子顺式作用元件、基因复制和共线性等进行了详细
分析。通过 RNA-seq 分析探索 AsARF 基因在白木香结香过程中的潜在机制，发现部分 AsARF 基因可能参与白木香
结香的防御和生长的动态平衡过程，可为深入了解白木香结香机制提供重要理论依据。 |
| 英文摘要: |
| 【Objective】Auxin response factors (ARF) are key transcription factors engaged in the auxin signaling system
and play significant roles in plant flower and fruit development, organ morphogenesis, hormone control, and stress responses. The
study aims to investigate the biological functions of AsARF gene in Aquilaria sinensis (Lour.) Gilg during the aroma formation
process.【Method】The AsARF genes in A. sinensis at genome-wide level were identified and analyzed by bioinformatics
approaches. The expression patterns of ARF family members in response to mechanical damage in A. sinensis were revealed
through transcriptome data analysis.【Result】Twenty-one AsARF genes were identified at the genome-wide level, and thesystematic bioinformatics analysis results showed that these 21 AsARF genes were divided into five groups, with gene sequences
within the same group sharing similar gene structures and conserved protein domains, while different groups of AsARF genes
exhibited significant variations in the number of exons and introns. Furthermore, cis-acting element analysis revealed that the
promoter regions of ARF genes in A. sinensis contained numerous sequences associated with sunlight, anaerobic conditions,
gibberellin and other response elements. Gene duplication and collinearity analysis indicated that fragment duplication events
played a crucial role in the evolution and expansion of the A. sinensis ARF gene family. Comparing with rice ARF genes, it
revealed there was a closer relationship between A. sinensis and Arabidopsis thaliana ARF genes. Additionally, transcriptome
data analysis demonstrated a significant decrease in expression levels of AsARF5, AsARF6, AsARF19, AsARF20, and
AsARF21 genes in response to mechanical damage in A. sinensis.【Conclusion】The study conducted the first genome-wide
identification of the AsARF gene family and offered a comprehensive analysis on their physicochemical properties, systematic
evolution, gene structure, conserved protein domains, promoter cis-acting elements, gene duplication, and collinearity.
Furthermore, the potential mechanisms of AsARF genes in the aroma formation process of A. sinensis were explored through
RNA-seq analysis. The results suggested that some AsARF genes may be involved in the defense and dynamic balance of aroma
formation in A. sinensis. These findings provided important theoretical basis for a deeper understanding of the aroma formation
mechanism in A. sinensis. |
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