| 何 磊 1
，刘风民 2
，冯月燕 3
，王梓鉴 1
，陈纪言 1
，张伟丽1,3.基于表型和分子标记分析 24 份巴戟天遗传多样性[J].广东农业科学,2024,51(5):56-68 |
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基于表型和分子标记分析 24 份巴戟天遗传多样性 |
| Analysis of Genetic Diversity of 24 Morinda offi cinalis How. Based on Phenotypic and Molecular Markers |
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| DOI：10.16768/j.issn.1004-874X.2024.05.005 |
| 中文关键词: 关键词：巴戟天 主成分分析 SRAP 分子标记 CDDP 分子标记 遗传多样性 |
| 英文关键词: Morinda offi cinalis How. principal component analysis SRAP molecular marker CDDP molecular marker genetic diversity |
| 基金项目:广东省现代产业园项目；德庆县科学技术局资助项目（B72020173） |
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| 中文摘要: |
| 【目的】广东省德庆县是道地药材巴戟天的产地，该文对广东德庆不同地区的 24 份巴戟天进行了种质资源的遗传多样性分析。【方法】以德庆县各地收集来的 24 份巴戟天资源为研究对象，使用主成分分析和SRAP、CDDP 两种分子标记方法对 24 份巴戟天材料进行遗传多样性分析。【结果】在表型分析中，基于主成分分析的聚类图发现，官圩镇和高良镇、凤村镇和高良镇分别聚类至 4 个类群中。此外，18 对 SRAP 引物组合和 16 条CDDP 引物扩增后分别获得 100 条和 98 条总条带，其中 SRAP 多态性条带为 50 条，多态性比率达 50%，平均遗传相似系数（GS）为 0.87，遗传相似系数变化范围为 0.71~0.97；CDDP 多态性条带为 80 条，条带之间多态性比率达79.0%， 各种质间遗传系数范围为0.71~0.96，平均遗传相似系数为 0.86，且当系数为 0.82 时，两种分子标记结果均表明高良镇、官圩镇和凤村镇的种质之间具有一定的相似性。【结论】结合表型和分子标记聚类结果，高良镇和官圩镇、高良镇和凤村镇的亲缘关系最近，SRAP 和 CDDP 两种分子标记方法对巴戟天遗传多样性的结果具有一定的差异，而联合分析可以更好地反映巴戟天遗传多样性研究及其资源的系统发育关系。 |
| 英文摘要: |
| 【Objective】Deqing County, Guangdong Province is a native habitat of Morinda offi cinalis How., a genuine
medicinal herb in China. In the study, the genetic diversity of germplasm resources of 24 varieties of Morinda offi cinalis How.
were analyzed.【Method】A total of 24 Morinda offi cinalis How. resources collected from Deqing County as research objects,
and principal component analysis and molecular marker methods (SRAP and CDD) were used to analyze the genetic diversity
of 24 Morinda offi cinalis How. materials.【Result】Phenotypic analysis showed that, in the cluster map based on principal
component analysis, Guanwei Town, Gaoliang Town, Fengcun Town and Gaoliang Town were clustered in four groups. In
addition,100 and 98 total bands were obtained in 18 pairs of SRAP primer combinations and 16 CDDP primers, among them,there were 50 SRAP polymorphic bands, resulting in a polymorphism ratio of 50%. The average genetic similarity coefficient (GS)
was 0.87, and the GS ranged from 0.71 to 0.97. There were 80 polymorphic bands in CDDP, with a polymorphism ratio of 79.0%.
The GS among germplasm resources ranged from 0.71 to 0.96, with an average (GS) of 0.86. When the coefficient was 0.82, both
SRAP and CDDP molecular markers showed certain similarities among the germplasm resources of Gaoliang Town, Guanxu
Town and Fengcun Town.【Conclusion】Combined with the clustering results of phenotypic and molecular marker, the genetic
relationship is closest between Gaoliang Town and Guanwei Town as well as Gaoliang Town and Fengcun Town. The results of
SRAP and CDDP molecular marker methods have certain differences in the genetic diversity of Morinda offi cinalis How., and
the combined analysis can better reflect the genetic diversity of Morinda offi cinalis How. and the phylogenetic relationship of
Morinda offi cinalis How. resources. |
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