| 孙军航 1
，罗梅英 1
，李素倩 1
，冯 鑫 1
，张辉华 1
，孙瑞萍 2
，柒启恩1.屎肠球菌细胞外囊泡对小鼠生长性能、肠道形态和粪便菌群的影响[J].广东农业科学,2024,51(5):135-143 |
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屎肠球菌细胞外囊泡对小鼠生长性能、肠道形态和粪便菌群的影响 |
| Effects of Enterococcus faecium Extracellular Vesicles on Growth Performance, Intestinal Morphology and Fecal Microbiota in Mice |
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| DOI：10.16768/j.issn.1004-874X.2024.05.012 |
| 中文关键词: 屎肠球菌 细胞外囊泡 小鼠 肠道形态 粪便菌群 免疫 |
| 英文关键词: Enterococcus faecium extracellular vesicle mice intestinal morphology fecal microbiota immunity |
| 基金项目:国家自然科学基金（31902228）；广东省重点建设学科科研能力提升项目（2022ZDJS040）；佛山科学技
术学院学科建设项目（CGZ0400162） |
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| 中文摘要: |
| 【目的】探究屎肠球菌细胞外囊泡（Enterococcus faecium extracellular vesicles，Efm-EV）对小鼠生长
性能、免疫、肠道形态和粪便菌群的影响，为屎肠球菌作为微生物饲料添加剂在动物生产中的推广应用提供理论依
据。【方法】将 20 只雄性 C57BL/6 小鼠随机分为对照组和试验组，每组 10 个重复，每个重复 1 只小鼠。试验期为
5 d，试验组小鼠每隔 1 d 灌胃含有 500 μg 的 Efm-EV 悬液，每只灌胃 150 μL；对照组小鼠灌胃等量 PBS 溶液。记
录小鼠每天的采食量、试验开始和结束时的体重，计算小鼠的平均日增重、平均日采食量、料重比 。【结果】与
PBS 处理相比，Efm-EV 处理小鼠的终末体重 、平均日增重、平均日采食量、料重比没有显著变化。Efm-EV 处理
小鼠的血清 IgA 和 IgG 水平与 PBS 处理小鼠相比显著下降（P ＜ 0.05），而小鼠血清 IL-10 水平显著提升（P ＜ 0.05），
TNF-α 无显著变化。与 PBS 处理相比，Efm-EV 处理小鼠十二指肠绒隐比显著提高（P ＜ 0.05），而小鼠十二指肠
绒毛高度、隐窝深度，空肠和回肠的绒毛高度、隐窝深度、绒隐比无明显变化。灌胃 Efm-EV 处理小鼠粪便菌群在
门水平上提高了厚壁菌门（Firmicutes）的细菌数量，降低了拟杆菌门（Bacteroidetes）的细菌数量；在科水平上提
高了毛螺菌科（Lachnospiraceae）的丰度，降低了 Muribaculaceae 科的丰度；在属水平上显著提高了小鼠瘤胃球菌
科 UCG009 （Ruminococcaceae UCG 009）的丰度（P ＜ 0.05）。【结论】灌胃 Efm-EV 能够提高小鼠机体的抗炎水
平，改善小鼠十二指肠形态结构，提高瘤胃球菌科 UCG009 的相对丰度。 |
| 英文摘要: |
| 【Objective】The study aimed to explore the effects of Enterococcus faecium extracellular vesicles (EfmEV) on growth performance, immunity, intestinal morphology and fecal microbiota in mice, providing theoretical basis for the
application of E. faecium as a microbial feed additive in animal production.【Method】A total of 20 male C57BL/6 micewere randomly divided into control group and experimental group with 10 replicates per group and 1 mouse per replicate.
The experiment lasted for 5 days. Mice in the experimental group were given 500 μg Efm-EV suspension every other day by
intragastric administration, with 150 μL for each mouse, and mice in the control group were given the same volume of PBS.
Daily feed intake, and body weight at the beginning and end of the experiment of each mouse were recorded, average daily gain
(ADG), average daily feed intake (ADFI) and feed/gain (F/G) of mice were calculated accordingly.【Result】Compared with
the PBS treatment group, no significant change was observed in the final body weight (FBW), ADG, ADFI and F/G of mice in
the Efm-EV treatment group. The serum immunoglobulin A (IgA) and immunoglobulin G (IgG) levels in the Efm-EV group
were significantly decreased compared with those in PBS treatment group (P ＜ 0.05), while the serum interleukin-10 (IL-
10) level was significantly increased (P ＜ 0.05), and there was no significant difference in serum tumor necrosis factor-α
(TNF-α) level. Compared with the PBS treatment, Efm-EV treatment significantly increased duodenal villus height/crypt depth
ratio (VH/CD) (P ＜ 0.05). There were no significant changes in villus height (VH), crypt depth (CD) of duodenum, and VH,
CD and VH/CD of jejunum and ileum. Efm-EV intragastric administration increased the number of Firmicutes and decreased
the number of Bacteroidetes at the phylum level, increased the abundance of Lachnospiraceae and decreased the abundance
of Muribaculaceae at the family level, significantly increased the abundance of Ruminococcaceae UCG009 at the genus level
(P＜ 0.05).【Conclusion】Intragastric administration of Efm-EV can improve the anti-inflammatory level, the duodenal
morphology and the relative abundance of Ruminococcaceae UCG009 in mice. |
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