MS-222 和丁香酚胁迫对四指马鲅幼鱼鳃及肝脏组织微观结构的影响

谢希尧 1; 2; 区又君 1; 徐淑玉1; 3; 林 欣 1; 2; 李加儿 1; 肖裕杰 2

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谢希尧 1，2，区又君 1 ，徐淑玉1，3，林欣 1，2，李加儿 1 ，肖裕杰 2.MS-222 和丁香酚胁迫对四指马鲅幼鱼鳃及肝脏组织微观结构的影响[J].广东农业科学,2024,51(5):144-154

查看全文 HTML MS-222 和丁香酚胁迫对四指马鲅幼鱼鳃及肝脏组织微观结构的影响

Effects of MS-222 and Eugenol Stress on Microstructure of Gill and Liver Tissue in Juvenile Eleutheronema tetradactylum

DOI：10.16768/j.issn.1004-874X.2024.05.013

中文关键词: 四指马鲅 MS-222 丁香酚麻醉鳃组织肝脏组织

英文关键词: Eleutheronema tetradactylum MS-222 eugenol anesthesia gill tissue liver tissue

基金项目:：广东省乡村振兴战略专项资金种业振兴项目（2022-SPY-00-013）；中国水产科学研究院基本科研业务费资助项目（2023TD58）

作者	单位
谢希尧 1，2，区又君 1 ，徐淑玉1，3，林欣 1，2，李加儿 1 ，肖裕杰 2	1. 中国水产科学研究院南海水产研究所 / 农业农村部南海渔业资源开发利用重点实验室，广东广州 510300； 2. 大连海洋大学水产与生命学院，辽宁大连 116000 ；3. 上海海洋大学水产与生命学院，上海 201306

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中文摘要:

【目的】探究不同质量浓度的 MS-222 和丁香酚胁迫对四指马鲅（Eleutheronema tetradactylum）幼鱼鳃和肝脏组织损伤的影响。【方法】设置 7 个浓度的 MS-222（20、30、35、40、45、50、60 mg/L）和 7 个浓度的丁香酚（5、10、20、25、30、40、50 mg/L），分析不同处理对平均全长 4.5（±0.5）cm、平均体质量 1.5（±0.5）g 的四指马鲅幼鱼的静水麻醉效应。【结果】当 MS-222 浓度不小于 35 mg/L 时，麻醉 467 s 后幼鱼开始出现应激反应；浓度为 50 mg/L 时可将部分幼鱼诱导进入麻醉期，浓度为 60 mg/L 时可在 293 s 后将全部幼鱼诱导进入麻醉期。丁香酚浓度不小于 25 mg/L 时，麻醉 291 s 后幼鱼开始出现应激反应；浓度为 30 mg/L 时可将部分幼鱼诱导进入麻醉期；浓度为 40、50 mg/L 时，分别可以在 194 s 和 102 s 将全部幼鱼诱导进入麻醉期。随着 MS-222 和丁香酚浓度的升高，幼鱼进入同一麻醉时期的时间显著缩短（P ＜ 0.05），高浓度下四指马鲅幼鱼进入麻醉时期的时间过快，超过耐受值可能对鱼体产生一定毒害作用，导致鱼体死亡。观察幼鱼组织病理切片发现，随着 MS-222 和丁香酚浓度的升高，幼鱼肝细胞出现空泡，排列紊乱，核内可见核溶解，肝板结构不清；鳃小片弯曲，末端肿大，严重者可胀破、脱落。【结论】适度的麻醉剂量可以降低鱼体对外界环境的应激反应，减少耗氧，不同浓度的 MS-222 和丁香酚（5 mg/L 除外）均可在 3 min 内对四指马鲅幼鱼产生镇静作用，当 2 种麻醉剂浓度过高、麻醉时间过长以及幼鱼长时间处于应激状态时，会出现死亡现象。

英文摘要:

【Objective】The effects of different mass concentrations of MS-222 and eugenol stresses on gill and livertissue damage in juvenile Eleutheronema tetradactylum were studied.【Method】Seven different mass concentrations of MS- 222 (20, 30, 35, 40, 45, 50 and 60 mg/L) and seven different mass concentrations of eugenol (5, 10, 20, 25, 30, 40 and 50 mg/L) were set to analyze the static anaesthesia effects of different treatments on juvenile E. tetradactylum with an average length of 4.5 (±0.5) cm and an average body weight of 1.5 (±0.5) g.【Result】When the concentration of MS-222 was not less than 35 mg/ L, the juvenile fish began to show stress reactions after 467 s of anesthesia. When the concentration was 50 mg/L, some juvenile fish could be induced into the anesthesia period. When the concentration was 60 mg/L, all juvenile fish could be induced into the anesthesia period after 293 s. When the concentration of eugenol was not less than 25 mg/L, the juvenile fish began to exhibit stress reactions after 291 s of anaesthesia; when the concentration was 30 mg/L, some juvenile fish could be induced into the anaesthesia period; when the concentrations were 40 and 50 mg/L, all juvenile fish could be induced into the anaesthesia period at 194 s and 102 s, respectively. With the increase of MS-222 and eugenol concentrations, the time for juvenile fish to enter the same anaesthesia period was significantly shortened (P ＜ 0.05). At high concentrations, the time for juvenile fish to enter the anaesthesia period was too fast, which exceeded the tolerance value and may have a certain toxic effect on the fish body, leading to fish death. By observing the pathological sections of juvenile fish tissues, it was found that, with the increase of MS-222 and eugenol concentrations, the liver cells of juvenile fish showed vacuoles, disordered arrangement, nuclear dissolution visible in the nucleus and unclear liver plate structure. The gill lamellae were curved with enlarged ends, and they may burst or fall off in severe cases. 【Conclusion】A moderate dosage of anaesthetic can reduce the stress response of fish to the external environment and reduce oxygen consumption. Different concentrations of MS-222 and eugenol (except for 5 mg/L) have a sedative effect on E. tetradactylum within 3 minutes. When the concentrations of the two anaesthetics are too high, the anaesthesia time is too long, and the juvenile fish are in a state of stress for a long time, the fish will die.

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