| 罗振亚 1
，徐 淑 1
，林开春２.枯草芽孢杆菌 Yz 菌株丸化种衣剂配方筛选试验[J].广东农业科学,2019,46(7):100-106 |
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枯草芽孢杆菌 Yz 菌株丸化种衣剂配方筛选试验 |
| Screening of Formula for Seed Pelleting Agentof Bacillus subtilis Yz strain |
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| DOI：10.16768/j.issn.1004-874X.2019.07.015 |
| 中文关键词: 枯草芽孢杆菌 棉花 抑菌活性 助剂 发芽率 生物种衣剂 |
| 英文关键词: Bacillus subtilis cotton inhibition activity additives germination rate biological seed-coating agent |
| 基金项目:广东省农业技术研发项目农业面源污染防控与产地环境安全共性技术创新团队（2018LM2151） |
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| 中文摘要: |
| 【目的】明确枯草芽孢杆菌 Yz 菌株发酵原粉对棉花苗期病害的抑菌活性，并以活芽孢下降率、
棉花种子发芽率为指标筛选 Yz 菌株发酵原粉的丸化种衣剂配方。【方法】采用对峙培养法测定 Yz 菌株发酵原
粉对棉花立枯病、炭疽病和红腐病的抑菌活性；采用 L9（33）正交试验，测定不同助剂对 Yz 菌株发酵原粉活
芽孢下降率及棉花种子发芽率的影响，筛选最佳丸化种衣剂配方。【结果】枯草芽孢杆菌 Yz 菌株发酵原粉 50
倍稀释液对棉花立枯病、棉花炭疽病和棉花红腐病的校正抑菌圈直径分别为 10.07、15.54、8.96 mm，随原粉浓
度的降低，抑菌圈直径逐渐减小。最佳助剂分别为硅藻土、羧甲基纤维素钠、木质素磺酸钠。【结论】枯草芽
孢杆菌 Yz 菌株发酵原粉对棉花立枯病、棉花炭疽病和棉花红腐病均有较好的抑菌活性。硅藻土、羧甲基纤维
素钠、木质素磺酸钠和 Yz 原粉的最佳组合比例为 3∶0.8∶0.1∶1。 |
| 英文摘要: |
| 【Objective】This study aims to clarify the bacteriostatic activity of the fermentation powder of Bacillus
subtilis Yz strain on cotton seedling diseases, and screens the formula for seed pelleting agent with the decline rate of live
spores and the germination rate of cotton seeds as indicators.【Method】The antibacterial activity of Yz strain fermentation
powder against cotton damping-off, cotton anthracnose, and cotton red rot was determined by the confrontation culture
method. In addition, by orthogonal design L9(33), the effects of different additives on the decline rate of live spores of
fermentation powder of B. subtilis Yz strain and the germination rate of cotton seeds were determined to screen the best
formula for seed pelleting agent.【Result】The corrected inhibitory zone diameter of fermentation powder of B. subtilis
Yz strain with 50 times dilution for cotton damping-off, cotton anthracnose, and cotton red rot were 10.07mm, 15.54mm
and 8.96mm, respectively. And the diameter of inhibitory zone decreased gradually with the decrease of the concentration
of fermentation powder. The best additives were diatomite, sodium carboxymethyl cellulose and sodium lignosulfonate.
【Conclusion】The fermentation powder of B. subtilis Yz strain show good antibacterial activity against cotton dampingoff,
cotton anthracnose, and cotton red rot. The optimum proportion is 3(diatomite) ∶ 0.8(sodium carboxymethyl
cellulose) ∶ 0.1(sodium lignosulfonate) ∶ 1(Yz fermentation powder). |
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