水稻细菌性条斑病菌侵染后抗、感近等基因系内源激素含量的变化

何圣贤; 万　瑶; 覃雪梅; 张　慧; 刘　芳

文章摘要

何圣贤，万　瑶，覃雪梅，张　慧，刘　芳.水稻细菌性条斑病菌侵染后抗、感近等基因系内源激素含量的变化[J].广东农业科学,2020,47(3):90-96

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Changes of Endogenous Hormone Content in Resistant and Susceptible Near-isogenic lines of Rice after Infection with Xanthomonas oryzae pv.oryzicola

DOI：10.16768/j.issn.1004-874X.2020.03.012

中文关键词: 水稻细菌性条斑病内源激素近等基因系

英文关键词: rice bacterial leaf streak endogenous hormones near-isogenic lines

基金项目:国家自然科学基金（ 31460341）; 广西自然科学基金（2019GXNSFAA185043）；广西科技计划项目（桂AB16380093）

作者	单位
何圣贤，万　瑶，覃雪梅，张　慧，刘　芳	广西大学农学院 / 亚热带农业生物保护与利用国家重点实验室，广西南宁 530004

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中文摘要:

【目的】研究水稻抗、感近等基因系与细菌性条斑病（简称细条病）病菌互作过程中内源激素含量的变化，为研究水稻细条病抗性机制提供理论依据。【方法】以水稻抗病近等基因系 LR19 和感病近等基因系 LS19 为材料，用针刺法接种细条病菌（处理组），对照组则用无菌水模拟接菌。接菌后 0、24、48、96 h 取水稻分蘖期叶片为样品，分析水杨酸（SA）、茉莉酸（JA）、乙烯（ETH）、赤霉素（GA）和玉米素（ZT）含量的变化。【结果】接菌后，LR19 处理组 SA、ETH、ZT 含量分别为 8.32 μg/mL、220.06 nmol/L 和 5.56 pmol/L，对照组则分别为 8.31 μg/mL、250.86 nmol/L 和 6.40 pmol/L。LS19 处理组 SA、ETH、ZT 含量分别为 6.57 μg/mL、 128.35 nmol/L 和 4.37 pmol/L，对照组则分别为 6.27 μg/mL、134.7 nmol/L 和 4.96 pmol/L。对于这 3 种激素，抗、感材料在处理组和对照组间差异均不显著，但是抗性材料的激素含量始终高于感性材料。接菌后，LR19 处理组 JA、GA 含量分别为 2 316.0、63.81 pmol/L，对照组则分别为 1 174.33、75.19 pmol/L，LS19 处理组 JA、GA 含量分别为 3 043.08、83.20 pmol/L，对照组则分别为 971.83、66.27 pmol/L。对于这 2 种激素，抗、感材料在处理组和对照组间差异显著，且抗性材料的激素含量低于感性材料。【结论】抗病近等基因系内源激素 SA、ETH、ZT 含量比感病近等基因系高，细条病菌的侵染会引起抗、感近等基因系叶片内激素 JA、GA 含量发生变化，参与水稻对细条病的抗性反应。

英文摘要:

【Objective】The objective was to study the changes of endogenous hormone content during the interaction between rice resistance, susceptible near-isogenic lines and pathogenic bacteria of Xanthomonas oryzae pv.oryzicola （Xooc）to provide theoretical basis for the study of the resistance mechanism of bacterial leaf streak.【Method】Using rice resistant and susceptible near isogenic lines（LR19 and LS19）as materials, the treatment groups were inoculated by pricking with pathogenic bacteria of Xooc, while the control groups were inoculated with sterilized water. At 0, 24, 48 and 96 h after inoculation, the leaves of rice at the tillering stage were taken as samples to analyzed the changes in the content of salicylic acid（SA）, jasmonic acid（JA）, ethylene（ETH）, gibberellin（GA）and zeatin（ZT）.【Result】After inoculation, the average contents of SA, ETH and ZT in the treatment groups of LR19 were 8.32 μg/mL 220.06 nmol/L and 5.56 pmol/ L, respectively, while those in the control groups were 8.31 μg/mL 250.86 nmol/L and 6.4 pmol/L, respectively. The average contents of SA, ETH and ZT in the treatment groups of LS19 were 6.57 μg/mL, 128.35 nmol/L and 4.37 pmol/L, respectively, while those in the control groups were 6.27 μg/mL, 134.7 nmol/L and 4.96 pmol/L, respectively. For these three hormones, there were no significant differences between the treatment and control groups of both lines, but the contents in resistant material were always higher than those in susceptible material. After inoculation, the average contents of JA and GA in the treatment groups of LR19 were 2 316.0 pmol/L and 63.81 pmol/L, respectively, while those in the control groups were 1 174.33 pmol/L and 75.19 pmol/L, respectively. The average contents of JA and GA in the treatment groups of LS19 were 3 043.08 pmol/L and 83.20 pmol/ L, respectively, while those in the control groups were 971.83 pmol/L and 66.27 pmol/L, respectively. For these two hormones, there were significant differences between the treatment and control groups of both lines, and the contents in resistant material were lower than those in susceptible material.【Conclusion】The contents of hormones SA, ETH and ZT in the resistant near isogenic lines were higher than those in the susceptible near-isogenic lines. Infection with Xooc induced the changes of JA and GA contents in both lines, which may be related to the BLS resistance.

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