| 李 英，吴庆华，杨华林，李 利，余知和，王 允.阿魏酸对抗生素应激小鼠血清抗氧化性
及其肠道微生物的影响[J].广东农业科学,2021,48(6):116-125 |
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阿魏酸对抗生素应激小鼠血清抗氧化性
及其肠道微生物的影响 |
| Effects of Ferulic Acid on Serum Antioxidant Capacity and Intestinal Microflora in Antibiotic Stressed Mice |
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| DOI：10.16768/j.issn.1004-874X.2021.06.016 |
| 中文关键词: 阿魏酸 抗生素 高通量测序 抗氧化 肠道菌群 |
| 英文关键词: ferulic acid antibiotic high-throughput sequencing antioxidation intestinal microflora |
| 基金项目:国家自然科学基金青年基金（31601679） |
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| 中文摘要: |
| 【目的】研究阿魏酸对抗生素应激小鼠的血清抗氧化性及其肠道微生物群落多样性的影响，
为阿魏酸微生态制剂研制提供理论基础。【方法】将小鼠分为对照组、抗生素组、抗生素 + 阿魏酸组，
通过各种试剂盒检测小鼠血清中的生化和氧化相关酶类指标，并利用高通量测序分析其肠道微生物群落。
【结果】阿魏酸使小鼠的 T-SOD 和 GSH-PX 活性比对照组分别提高 20.50% 和 26.27%，并使小鼠的总抗氧化能
力比抗生素组提高 56.36%，同时通过 NOS 基因下调信号分子促进肠道消化。抗生素导致小鼠肠道微生物的丰度
和多样性显著下降，阿魏酸对该现象无显著干预，但阿魏酸组和抗生素组小鼠分别有 215、259 个独有的操作分
类单元，可见阿魏酸对肠道群落组成造成了显著影响。阿魏酸通过上调壁厚菌门与变形菌门在门水平上的丰度比，
并通过促进梭菌属、芽胞八叠球菌属和变形杆菌属等增殖，防止条件致病菌肠球菌属过度繁殖，改善了肠道的
群落组成。【结论】阿魏酸能够提高小鼠血清的抗氧化能力，改变肠道群落结构，通过多方面改善抗生素对小
鼠肠道的代谢和炎症反应产生负面的影响。 |
| 英文摘要: |
| 【Objective】Effects of ferulic acid on serum antioxidant capacity and intestinal microflora diversity in
antibiotic stressed mice were studied for providing a theoretical basis for the development of ferulic acid microecological
preparations.【Method】The mice were divided into control group, antibiotic group and antibiotic + ferulic acid group.
Biochemical and oxidation related enzymes in serums of mice were detected by various kits, and their effects on intestinal
microbial community were analyzed by high-throughput sequencing.【Result】Ferulic acid increased the activities of
T-SOD and GSH-Px of mice by 20.50% and 26.27% respectively, and increased the total antioxidant capacity by 56.36%
compared with that of antibiotic group. At the same time, ferulic acid promoted the intestinal digestion through NOS downregulated signal molecules. Antibiotic led to significant decrease of the abundance and diversity of intestinal microflora
in mice, while ferulic acid had no significant interference on this phenomenon. However, there were 215 and 259 unique
OTU in the ferulic acid group and the antibiotic group, respectively. Therefore, ferulic acid had a significant effect on the
composition of intestinal microflora. Ferulic acid optimized the intestinal microflora composition through up-regulating the ratio of Firmicutes to Proteobacteria, promoting the proliferation of Clostridium, Sporosarcina and Proteus and preventing the
over reproduction of Enterococcus. 【Conclusion】Ferulic acid can improve the antioxidant capacity of mice serum, change the
intestinal microflora composition and improve the metabolism and inflammatory reaction of intestinal tract in mice through many
ways which affected by antibiotics. |
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