| 李落叶，邓 苗，黄少彬，李 樨，杨童童，柯碧英.药用植物厚朴 TRAP 标记的开发和利用[J].广东农业科学,2022,49(6):21-27 |
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药用植物厚朴 TRAP 标记的开发和利用 |
| Development and Utilization of TRAP Molecular Markerson Medicinal Plant Magnolia offi cinalis |
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| DOI：10.16768/j.issn.1004-874X.2022.06.003 |
| 中文关键词: 药用植物 厚朴 靶区域扩增多态性标记 微卫星标记 遗传多样性 |
| 英文关键词: medicinal plant Magnolia offi cinalis Target Region Amplified Polymorphism (TRAP) Simple Sequence
Repeat (SSR) genetic diversity |
| 基金项目:广东省林业科技创新项目（2018KJCX025） |
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| 中文摘要: |
| 【目的】开发厚朴新型分子标记，分析广东乐昌龙山林场厚朴资源的亲缘关系，以方便后续进行
厚朴优株选择和优良品种选育等研究工作。【方法】以 14 个由广东乐昌龙山林场种植的、种源来自江西省各
地的药用植物厚朴为材料，根据 GenBank 中厚朴 cDNA 序列信息，利用 NCBI（https://www.ncbi.nlm.nih.gov）上
Primer BLAST 工具设计厚朴靶区域扩增多态性（Target Region Amplified Polymorphism, TRAP）标记固定引物，与
SRAP（Sequence Related Amplified Polymorphism）随机引物配对，扩增厚朴基因组 DNA，开发厚朴 TRAP 标记。
统计 TRAP 标记与厚朴 SSR（Simple Sequence Repeat）标记扩增产物的电泳带型图谱，用 NTSYSpc2.10e 软件进
行聚类分析，分析 14 个厚朴资源的遗传多样性及其亲缘关系。【结果】 从 14 条固定引物与 5 条随机引物配组
形成的 70 对引物中共开发出 7 个扩增产物电泳条带清晰、带型丰富、多态性好的厚朴 TRAP 标记；构建了厚朴
资源的亲缘关系树状图，结果表明龙山林场厚朴资源遗传相似系数在 0.75~0.88 之间，母株与对应子代之间遗传
相似系数并不是最高。【结论】厚朴群体内基因交流频繁，遗传多样性丰富，新开发的 TRAP 标记将为厚朴优
株鉴别、优良品种选育、分子标记辅助选择等后续研究工作提供便利。 |
| 英文摘要: |
| 【Objective】The study was carried out to develop new molecular markers and analyze the molecular
phylogeny of Magnolia officinalis in Longshan Tree Farm, Lechang, Guangdong, with a view to facilitating subsequent
researches on the selection of excellent plants and breeding of superior varieties.【Method】Fourteen Magnolia offi cinalis
(provenances from Jiangxi Province) individual plants collected from Longshan Tree Farm were used as tested materials. By
using Primer BLAST on NCBI (https://www.ncbi.nlm.nih.gov), fixed primers of TRAP molecular markers were designed based
on the sequences of M. offi cinalis cDNA in the GenBank. The fixed primers were matched with random primers (sequence
related amplified polymorphism) to amplify genomic DNA and develop TRAP molecular markers. The electrophoretogram of
TRAP markers and amplified products of M. offi cinalis SSR (Simple Sequence Repeat) were counted. Then, NTSYSpc2.10e
was used for cluster analysis to discover the genetic diversity and phylogeny of fourteen M. offi cinalis resources.【Result】
Seven TRAP molecular markers showing good polymorphism and clear electrophoresis bands were successfully developed
from seventy primer pairs formed by fourteen fixed primers and five random primers. The phylogenetic tree of M. offi cinalis plants was constructed. The genetic similarity coefficient of these M.offi cinalis plants was 0.75-0.88 and it was not the highest
between mother plant and its offspring.【Conclusion】M. officinalis was rich in genetic diversity and gene exchange was
frequent in M. offi cinalis population. The newly developed TRAP molecular markers will facilitate identification of superior
plants, breeding of excellent varieties and marker-assisted selection of M. offi cinalis in the future. |
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