| 周 炼，陈 洛，吴 伟，杨梯丰，张少红，赵均良.一个锌指结合蛋白编码基因调控水稻种子萌发[J].广东农业科学,2022,49(9):1-9 |
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一个锌指结合蛋白编码基因调控水稻种子萌发 |
| Seed Germination in Rice Regulated by A Zinc-finger Binding Protein Encoding Gene |
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| DOI：10.16768/j.issn.1004-874X.2022.09.001 |
| 中文关键词: 水稻 种子萌发 锌指结合蛋白 直播 过表达 低温 |
| 英文关键词: rice seed germination zinc-finger binding protein direct seeding overexpression low temperature |
| 基金项目:国家自然科学基金（32001613）；广东省自然科学基金（2022A1515012135，2022A1515012328）；广州市科技计划项目（202102021004） |
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| 中文摘要: |
| 【目的】致力于挖掘水稻低温萌发力（LTG）相关基因，并解析其分子作用机理，为水稻低温萌发力遗传改良和分子育种提供理论基础。【方法】利用已有的不同水稻品种低温萌发转录组测序数据筛选差异表达基因，构建目的基因的遗传转化材料，通过转基因材料的萌发试验鉴定目的基因功能。【结果】OsYIPL1 编码 Yippee 家族锌指结合蛋白，在高 LTG 品种中高表达，被选作深入研究的对象。时空表达结果显示，OsYIPL1在水稻花药和幼胚等组织中高表达。通过遗传转化获得两个过表达株系 OX-1、OX-2，其 T2代种子用于常温（28℃）和低温（13℃）萌发试验。当 OsYIPL1 表达量升高到野生型的 44.5 倍（OX-1）时，其种子在常温下的萌发率比野生型平均提高 18.3%；当 OsYIPL1 表达量升高到野生型的 88.9 倍（OX-2）时，其种子在常温下的萌发率比野生型平均降低 54.5%。低温下，过表达 OsYIPL1 均会降低种子的萌发力，OX-1 和 OX-2 的低温萌发率比对照平均降低 43.8% 和 81.5%。【结论】OsYIPL1 参与调控水稻种子萌发过程，但在不同温度下的作用差异显著，其分子作用机理仍待进一步研究。 |
| 英文摘要: |
| 【Objective】This study was committed to explore low-temperature germinability (LTG) related genes of rice, and clarify the molecular mechanism, which can provide theoretical foundation for genetic modification and molecular breeding of low-temperature germinability in rice.【Method】Based on the acquired RNA-seq data of low-temperature germination in different rice accessions, differentially expressed genes (DEGs) were screened out. Genetic transformation materials of the chosen DEG were constructed, which were applied for the identification of gene function through seed germination trials.【Result】A Yippee family member encoding gene OsYIPL1, which had a higher expression level in high LTG accessions, was chosen for further study. The results of temporal and spatial expression of OsYIPL1 showed that it was specifically expressed higher in rice anther and young embryo. Two over-expression lines of OsYIPL1, OX-1and OX-2, were acquired and the seeds from the T2 generation were applied for germination trials at normal temperature (28℃ ) and low temperature (13 ℃ ). The results showed that when the expression of OsYIPL1 was increased to 44.5 times that of the wild type (OX-1), the germination rate of its seeds at normal temperature was 18.3% higher than that of the wild type on average. When the expression of OsYIPL1 was increased to 88.9 times that of the wild type (OX-2), the germination rate of its seeds at normal temperature was 54.5% lower than that of the wild type. At low temperature, overexpression of OsYIPL1 could reduce seed germination, and the germination rates of OX-1 and OX-2 were 43.8% and 81.5% lower than those of the control on average.【Conclusion】OsYIPL1 participates in regulation of rice seed germination, but the effects are obviously different at different temperatures. The underlying mechanism is waiting for further research. |
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